Synaptophysin (SYP) antibody

Antigen: Synaptophysin (SYP)

Synonyms: Syn, p38, AI848995, A230093K24Rik, Syp1, syp-A, xsypi, MGC86277, syp-B, xsypii, MGC86267

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN197755

Additional Information

Alternative name: Synaptophysin

Immunogen: Synthetic human synaptophysin coupled to keyhole limpet hemocyanin.

Format: Liquid (prediluted)

Specificity: This antibody reacts with a hydrophilic peptide of 38 kD. The antibody stains neuroendocrine cells of adrenal medulla, carotid body, skin, pituitary gland, lung, and pancreas.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Pancreas. Cellular localization: Cytoplasmic

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Navone, Jahn, Di Gioia et al.: "Protein p38: an integral membrane protein specific for small vesicles of neurons and neuroendocrine cells." in: The Journal of cell biology, Vol. 103, Issue 6 Pt 1, pp. 2511-27, 1987 (PubMed).

Gould, Wiedenmann, Lee et al.: "Synaptophysin expression in neuroendocrine neoplasms as determined by immunocytochemistry." in: The American journal of pathology, Vol. 126, Issue 2, pp. 243-57, 1987 (PubMed).

Kayser, Schmid, Ebert et al.: "Expression of neuroendocrine markers (neuronspecific enolase, synaptophysin and bombesin) in carcinoma of the lung." in: Pathology, research and practice, Vol. 183, Issue 4, pp. 412-7, 1988 (PubMed).