Orexin A antibody (AA 33-124)

Catalog No. ABIN1980481

Product Details anti-Orexin A Antibody

Target: Orexin A (OXA)

Binding Specificity: AA 33-124

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Orexin A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Orexin A (OXA)

Specificity: The antibody is a rabbit polyclonal antibody raised against OXA. It has been selected for its ability to recognize OXA in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Orexin A (OXA) corresdonding to Gln33~Pro124 (Accession # O55241) with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Orexin A

Target: Orexin A (OXA)

Alternative Name: Orexin A (OXA Products)

Synonyms: PPOX antibody, orexin-A antibody, hypocretin antibody, hypocretin neuropeptide precursor antibody, Hcrt antibody, HCRT antibody

Background: Hypocretin-1

Pathways: Synaptic Membrane, Feeding Behaviour