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CD235a/GYPA antibody (FITC)

GYPA Reactivity: Human, Rat FACS Host: Mouse Monoclonal JC159 FITC
Catalog No. ABIN1981887
  • Target See all CD235a/GYPA (GYPA) Antibodies
    CD235a/GYPA (GYPA) (Glycophorin A (GYPA))
    Reactivity
    • 130
    • 17
    • 12
    • 9
    Human, Rat
    Host
    • 69
    • 63
    • 14
    • 1
    Mouse
    Clonality
    • 89
    • 56
    Monoclonal
    Conjugate
    • 58
    • 13
    • 12
    • 11
    • 7
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This CD235a/GYPA antibody is conjugated to FITC
    Application
    • 102
    • 57
    • 42
    • 30
    • 26
    • 24
    • 15
    • 13
    • 13
    • 13
    • 13
    • 5
    • 5
    • 5
    • 4
    • 3
    • 1
    • 1
    Flow Cytometry (FACS)
    Specificity
    The mouse monoclonal antibody JC159 recognizes an epitope between amino acids 27 and 40 of the extracellular portion of CD235a (glycophorin A), a sialoglycoprotein expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL. The antibody does not react with glycophorin B.
    Cross-Reactivity (Details)
    Human, Rat
    Purification
    Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
    Immunogen
    Membrane preparation from splenic hairy cell leukemia
    Clone
    JC159
    Isotype
    IgG1
    Top Product
    Discover our top product GYPA Primary Antibody
  • Application Notes
    Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
    Comment

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

    Restrictions
    For Research Use only
  • Buffer
    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze. Avoid prolonged exposure to light.
    Do not use after expiration date stamped on vial label.
    Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Yamauchi, Takenaka, Urata, Shima, Kikushige, Tokuyama, Iwamoto, Nishihara, Iwasaki, Miyamoto, Honma, Nakao, Matozaki, Akashi: "Polymorphic Sirpa is the genetic determinant for NOD-based mouse lines to achieve efficient human cell engraftment." in: Blood, Vol. 121, Issue 8, pp. 1316-25, (2013) (PubMed).

    Alijotas-Reig, Palacio-Garcia, Llurba, Vilardell-Tarres: "Cell-derived microparticles and vascular pregnancy complications: a systematic and comprehensive review." in: Fertility and sterility, Vol. 99, Issue 2, pp. 441-9, (2013) (PubMed).

    Maijenburg, Kleijer, Vermeul, Mul, van Alphen, van der Schoot, Voermans: "The composition of the mesenchymal stromal cell compartment in human bone marrow changes during development and aging." in: Haematologica, Vol. 97, Issue 2, pp. 179-83, (2012) (PubMed).

  • Target
    CD235a/GYPA (GYPA) (Glycophorin A (GYPA))
    Alternative Name
    CD235a (GYPA Products)
    Synonyms
    GYPA antibody, gpa antibody, AI853584 antibody, CD235a antibody, GPA antibody, GPErik antibody, GPSAT antibody, HGpMiV antibody, HGpMiXI antibody, HGpSta(C) antibody, MN antibody, MNS antibody, PAS-2 antibody, glycophorin A (MNS blood group) antibody, glycophorin A antibody, GYPA antibody, Gypa antibody
    Background
    Glycophorin A (MNS blood group),CD235a (glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to CD235b (glycophorin B, GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, a Streptococcus adhesin.,Glycophorin A, GYPA, GPA, PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein, GPSAT, GPErik, MSN blood group
    Gene ID
    2993
    UniProt
    P02724
    Pathways
    Maintenance of Protein Location
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