EPH Receptor B4 antibody (C-Term)

Catalog No. ABIN200838

Product Details anti-EPH Receptor B4 Antibody

Target: EPH Receptor B4 (EPHB4)

Binding Specificity: C-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EPH Receptor B4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Specificity: Recognizes human endogenous EphB4 receptor at ~110-120kD, with weak cross-reactivity with the EphB2 receptor.

Purification: Protein A purified

Immunogen: Recombinant protein derived from the C-terminal region of the human EphB4 protein.

Type of Immunogen: Recombinant protein

Clone: 3D7G8

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P, IP, WB (1 - 3 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 1-3 μg/mL. Immunoprecipitation: 5 μg/IP reaction. Immunohistochemistry: 10 μg/mL. Formalin-fixed, paraffin-embedded tissues require heat induced epitope retrieval (HIER) with citrate buffer,  pH 6.0 prior to staining. Positive control: Western Blot: EphB4-transfected 293T cells and human MDA-MB-231 breast carcinoma cell lysates.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for EPH Receptor B4

Target: EPH Receptor B4 (EPHB4)

Alternative Name: EPHB4 / EPH Receptor B4 (EPHB4 Products)

Synonyms: HTK antibody, MYK1 antibody, TYRO11 antibody, htk antibody, myk1 antibody, ephB4a antibody, tyro11 antibody, ephb4b antibody, MGC130997 antibody, AI042935 antibody, Htk antibody, MDK2 antibody, Myk1 antibody, Tyro11 antibody, cb646 antibody, ephB4 antibody, rtk5 antibody, EphB-rtk8 antibody, cb417 antibody, rtk8 antibody, EPH receptor B4 antibody, EPH receptor B4 L homeolog antibody, EPH receptor B4 S homeolog antibody, Eph receptor B4 antibody, eph receptor B4a antibody, eph receptor B4b antibody, EPHB4 antibody, ephb4.L antibody, ephb4.S antibody, ephb4 antibody, Ephb4 antibody, ephb4a antibody, ephb4b antibody

Background: Name/Gene ID: EPHB4
Subfamily: Ephrin Receptor
Family: Protein Kinase

Synonyms: EPHB4, EPH receptor B4, Ephrin receptor EphB4, Ephrin type-B receptor 4, HTK, MYK1, TYRO11, Soluble EPHB4 variant 1, Soluble EPHB4 variant 3, Hepatoma transmembrane kinase, MDK2, Soluble EPHB4 variant 2, Tyrosine-protein kinase TYRO11

Gene ID: 2050

UniProt: P54760

Pathways: RTK Signaling