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GM-CSF Protein

CSF2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 98 % as determined by(a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE silver-stained gel Active
Catalog No. ABIN2017902
  • Target See all GM-CSF (CSF2) Proteins
    GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Origin
    • 30
    • 13
    • 9
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 29
    • 11
    • 8
    • 5
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Sequence
    MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN
    Specificity
    UV Scan: The maximal absorption wave is 279+/-3 nm.
    Characteristics
    The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is less than 0.1 ng/mL, corresponding to a Specific Activity of 1.0x10^7 IU/mg.
    LKDFLLVIPF DCWEPVQETh e sequence of the first fifteen N-terminal amino acids has been found to be (Met)-Ala-Pro-Ala-Arg-Ser-Pro-Ser-Pro-Ser-Thr-Gln-Pro-Trp-Glu-His.
    Purity
    Greater than 98 % as determined by(a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE silver-stained gel
    Endotoxin Level
    < 0.03 ng/μg (0.3 IEU/μg) determined by LAL test
    Top Product
    Discover our top product CSF2 Protein
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    It is recommended that the lyophilized Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) be reconstituted in sterile 18 M omega-cm H2O not less than 100 μg/mL, which can then be further diluted to other aqueous solutions.
    Buffer
    The protein was lyophilized after extensive dialysis against 20 mM Phosphate buffer, pH 7.0, 150 mM NaCl, 5 % Mannitol buffer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    Lyophilized Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF), although stable at room temperature for three weeks, should be stored desiccated at -18 °C or below. Upon reconstitution, Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) may be stored at 4 °C for short periods (two to seven days). For long term storage, it is recommended that a carrier protein (0.1% HSA or BSA) be added.
  • Mathew, Zaineb, Verma: "GM-CSF-DFF40: a novel humanized immunotoxin induces apoptosis in acute myeloid leukemia cells." in: Apoptosis : an international journal on programmed cell death, Vol. 18, Issue 7, pp. 882-95, (2013) (PubMed).

    Wang, Thomson, Allan, Jackson, Olson, Hercus, Nero, Turner, Parker, Lopez, Waddell, Anderson, Hamilton, Schrader: "Characterization of pathogenic human monoclonal autoantibodies against GM-CSF." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 19, pp. 7832-7, (2013) (PubMed).

    Côté, Plante, Tardif, Tremblay: "Dectin-1/TLR2 and NOD2 agonists render dendritic cells susceptible to infection by X4-using HIV-1 and promote cis-infection of CD4(+) T cells." in: PLoS ONE, Vol. 8, Issue 7, pp. e67735, (2013) (PubMed).

    Bouchlaka, Sckisel, Chen, Mirsoian, Zamora, Maverakis, Wilkins, Alderson, Hsiao, Weiss, Monjazeb, Hesdorffer, Ferrucci, Longo, Blazar, Wiltrout, Redelman, Taub, Murphy: "Aging predisposes to acute inflammatory induced pathology after tumor immunotherapy." in: The Journal of experimental medicine, Vol. 210, Issue 11, pp. 2223-37, (2013) (PubMed).

    Allan, Stax, Zheng, Chung, Kozak, Tan, van den Elzen: "CD1d and CD1c expression in human B cells is regulated by activation and retinoic acid receptor signaling." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 9, pp. 5261-72, (2011) (PubMed).

    Lambert, Barabé, Gilbert, Tremblay: "DCIR-mediated enhancement of HIV-1 infection requires the ITIM-associated signal transduction pathway." in: Blood, Vol. 117, Issue 24, pp. 6589-99, (2011) (PubMed).

    Ciesielski, Ahluwalia, Munich, Orton, Barone, Chanan-Khan, Fenstermaker: "Antitumor cytotoxic T-cell response induced by a survivin peptide mimic." in: Cancer immunology, immunotherapy : CII, Vol. 59, Issue 8, pp. 1211-21, (2010) (PubMed).

  • Target
    GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
    Alternative Name
    Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) (CSF2 Products)
    Synonyms
    GMCSF Protein, GM-CSF Protein, CSF2 Protein, Csfgm Protein, Gm-CSf Protein, MGI-IGM Protein, Gm-csf Protein, Gmcsf Protein, CSF Protein, colony stimulating factor 2 Protein, colony stimulating factor 2 (granulocyte-macrophage) Protein, CSF2 Protein, Csf2 Protein
    Background
    Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) is produced by a number of different cell types, including activated T cells, B cells, macrophages, mast cells, endothelial cells, and fibroblasts, in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. On mature hematopoietic, monocytes/macrophages and eosinophils.Human Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) can induce human endothelial cells to migrate and proliferate. Additionally, Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) can stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma, and adenocarcinoma cell lines.
    Synonyms: rHuGM-CSF, GM-CSF
    Molecular Weight
    14,000 Da
    Pathways
    JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin
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