GDF2 antibody (AA 320-429)

Catalog No. ABIN203805

Product Details anti-GDF2 Antibody

Target: GDF2 (Growth Differentiation Factor 2 (GDF2))

Binding Specificity: AA 320-429

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GDF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut), Functional Studies (Func)

Specificity: Recognizes human Bone Morphogenetic Protein 9. Does not cross react with recombinant human BMP-1, -1.1, -2, -3, -3b, -4, -5, -6, -7, -8 or -10. In direct ELISAs, ~80 % cross-reactivity with recombinant mouse BMP-9 and 10 % cross-reactivity with recombinant mouse BMP-10 is observed.

Purification: Affinity purified

Sterility: 0.2 μm filtered

Immunogen: Recombinant protein corresponding to aa320-429 from human BMP-9, expressed in E.coli (UniProt# Q9UK05).

Type of Immunogen: Recombinant protein

Clone: 360107

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Func, Neut, WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot, ELISA and Neutralization. Western Blot: 1-2 μg/mL, The detection limit is ~5 ng/lane under non-reducing conditions. Direct ELISA: 0.5-1.0 μg/mL, The detection limit is ~5 ng/well. Neutralization of human BMP-9 bioactivity: The ND50 is defined as the concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when the cytokine is present at a concentration just high enough to elicit maximum response. The ND50 of this lot is ~0.01-0.04 μg/mL in the presence of 2 ng/mL rhBMP-9 using ATDC5 chondrogenic cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from a 0.2 um filtered solution in PBS, 5 % trehalose.

Handling Advice: Avoid frequent freeze thaws. Store undiluted.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -70°C. Stable for 1 year from date of receipt.

Target Details for GDF2

Target: GDF2 (Growth Differentiation Factor 2 (GDF2))

Alternative Name: GDF2 / BMP9 (GDF2 Products)

Synonyms: bmp9 antibody, BMP-9 antibody, BMP9 antibody, Bmp9 antibody, DSL1 antibody, growth differentiation factor 2 antibody, gdf2 antibody, GDF2 antibody, Gdf2 antibody

Background: Name/Gene ID: GDF2
Family: TGF beta

Synonyms: GDF2, BMP-9, BMP9, GDF-2, Bone morphogenetic protein 9

Gene ID: 2658

UniProt: Q9UK05

Pathways: Transition Metal Ion Homeostasis