PIP5K1C antibody (C-Term)

Catalog No. ABIN204569

Product Details anti-PIP5K1C Antibody

Target: PIP5K1C (Phosphatidylinositol-4-Phosphate 5-Kinase, Type I, gamma (PIP5K1C))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIP5K1C antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Specificity: Binds to a 85-90kD human protein. Binding can be blocked by the peptide immunogen.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human PIP5K1C.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:50000), Neut, WB

Usage: Western Blot. ELISA: 1:50000. ELISA: Antibody titers and specificity were analyzed by ELISA assay using control samples (varying concentration of GST-EPOr) and control anti GST antibody. Affinity purified antibody was found to detect EPOr at concentrations of 50 ng/mL. Neutralization of ligand binding has been detected using Erythroleukemia cell line TF-1 (3000 EPO receptors/cell) to block Iodine-labeled erythropoietin ligand binding. Western blot concentration used 10 μg/mL. It will recognize EPOr-GST at its migration of 49kD on SDS-PAGE. Good secondary anti-sheep reagents are essential for this assay. Recommended use of rabbit anti-sheep rather than donkey anti-sheep antibodies as a secondary labeling reagents.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 1 mg/mL BSA, 0.1 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for PIP5K1C

Target: PIP5K1C (Phosphatidylinositol-4-Phosphate 5-Kinase, Type I, gamma (PIP5K1C))

Alternative Name: PIP5K1C (PIP5K1C Products)

Synonyms: LCCS3 antibody, PIP5K-GAMMA antibody, PIP5Kgamma antibody, PIPKIg_v4 antibody, MGC130766 antibody, PIP5K1C antibody, AI115456 antibody, AI835305 antibody, Pip5kIgamma antibody, phosphatidylinositol-4-phosphate 5-kinase type 1 gamma antibody, phosphatidylinositol-4-phosphate 5-kinase, type I, gamma S homeolog antibody, phosphatidylinositol-4-phosphate 5-kinase, type I, gamma antibody, phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma antibody, PIP5K1C antibody, Pip5k1c antibody, pip5k1c.S antibody, pip5k1c antibody

Background: Name/Gene ID: PIP5K1C

Synonyms: PIP5K1C, Diphosphoinositide kinase, KIAA0589, LCCS3, PIP5K1-gamma, Type I PIP kinase, PIP5KIgamma, PtdIns(4)P-5-kinase 1 gamma, PIP5K-GAMMA, PIP5Kgamma, PIPKIg_v4

Gene ID: 23396

UniProt: O60331

Pathways: PI3K-Akt Signaling, Inositol Metabolic Process, Cell-Cell Junction Organization, Maintenance of Protein Location, Synaptic Vesicle Exocytosis