E2F1 antibody (AA 1-89, AA 342-386)

Catalog No. ABIN210874

Product Details anti-E2F1 Antibody

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Binding Specificity: AA 1-89, AA 342-386

Reactivity: Human, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Specificity: Recognizes human E2F-1 at 55kD. Species cross-reactivity: Xenopus.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig (100%) Rat, Elephant (94%) Opossum (89%) Chicken, Lizard (83%).

Purification: Protein G purified

Immunogen: GST fusion protein corresponding to full-length human E2F1 (Accession #NP_005216). Epitope: aa1-89 for clone KH20, aa342-386 for clone KH95.

Type of Immunogen: Fusion protein

Clone: KH20-KH95

Isotype: IgG

Application Details

Application Notes: Approved: IP, WB

Usage: Western Blot Analysis: 0.5-2 μg/mL of antibody detected E2F-1 (~55kD) in a HeLa nuclear extract. Positive Antigen Control: HeLa nuclear extract. Use 20 μg per lane. Immunoprecipitation: 4 μg of a previous lot of antibody immunoprecipitated E2F-1 from HeLa nuclear extract.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F1 (E2F1 Products)

Synonyms: CG6376 antibody, DRTF1/E2F antibody, DmE2F-1 antibody, Dmel\\CG6376 antibody, Dp antibody, E(Sev-CycE)3A antibody, E(var)3-93E antibody, E(var)3-95E antibody, E(var)93E antibody, E2F antibody, E2F-1 antibody, E2F1 antibody, E2f1 antibody, Evar(3)164 antibody, dE2F antibody, dE2F1 antibody, dE2f antibody, dE2f1 antibody, de2f1 antibody, def21 antibody, drosE2F1 antibody, e2f antibody, e2f1 antibody, l(3)07172 antibody, l(3)j3B1 antibody, l(3)j3C2 antibody, l(3)rM729 antibody, RBAP1 antibody, RBBP3 antibody, RBP3 antibody, mKIAA4009 antibody, xE2F antibody, ATE2FB antibody, E2F TRANSCRIPTION FACTOR-1 E2F1 antibody, E2F transcription factor 1 antibody, E2FB antibody, E2FB TRANSCRIPTION FACTOR antibody, E2F transcription factor 1 antibody, E2F transcription factor 1 L homeolog antibody, E2f1 antibody, E2F1 antibody, e2f1.L antibody

Background: Name/Gene ID: E2F1

Synonyms: E2F1, E2F transcription factor 1, PRB-binding protein E2F-1, RBAP-1, RBBP3, RBBP-3, Transcription factor E2F1, RBAP1, E2F-1, PBR3

Gene ID: 1869

NCBI Accession: NP_005216

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy