EHMT2 antibody (AA 71-88)

Catalog No. ABIN210887

Product Details anti-EHMT2 Antibody

Target: EHMT2 (Ankyrin Repeat-Containing Protein (EHMT2))

Binding Specificity: AA 71-88

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EHMT2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Recognizes human G9a protein, Mr ~140kD. Species cross-reactivity: Human. Predicted to cross-react with mouse based on sequence homology.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%) Mouse, Rat, Elephant, Panda, Bovine, Horse, Rabbit, Pig (94%).

Purification: Protein A purified

Immunogen: KLH-conjugated, synthetic peptide (C-DERVDSDSKSEVEALTEQ) corresponding to amino acids 71-88 of human G9a, with an N-terminal cysteine added for conjugation purposes. The immunizing sequence has 17/18 identical amino acids in mouse. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%), Mouse, Rat, Elephant, Panda, Bovine, Horse, Rabbit, Pig (94%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 2 μg/mL)

Usage: Suitable for use in Western Blot. Western Blot: 0.2-2 μg/mL detected G9a in HeLa nuclear extract cell lysate. HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-G9a (0.5 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for EHMT2

Target: EHMT2 (Ankyrin Repeat-Containing Protein (EHMT2))

Alternative Name: EHMT2 / G9A (EHMT2 Products)

Synonyms: BAT8 antibody, C6orf30 antibody, G9A antibody, GAT8 antibody, KMT1C antibody, NG36 antibody, T27C4.12 antibody, T27C4_12 antibody, DL3235W antibody, FCAALL.188 antibody, T18B16.120 antibody, T18B16_120 antibody, F9H3.13 antibody, F9H3_13 antibody, F9H3.7 antibody, F9H3_7 antibody, F9H3.9 antibody, F9H3_9 antibody, F9H3.6 antibody, F9H3_6 antibody, F13G24.40 antibody, T20K14.110 antibody, T20K14_110 antibody, MWD22.10 antibody, MWD22_10 antibody, MRB17.12 antibody, MRB17_12 antibody, MAF19.22 antibody, MAF19_22 antibody, Bat8 antibody, D17Ertd710e antibody, G9a antibody, Ng36 antibody, euchromatic histone lysine methyltransferase 2 antibody, ankyrin repeat-containing protein antibody, Ankyrin repeat family protein antibody, euchromatic histone lysine N-methyltransferase 2 antibody, EHMT2 antibody, ank1 antibody, IscW_ISCW006888 antibody, Arnit_2917 antibody, AT3G04470 antibody, AT4G14390 antibody, AT4G19150 antibody, AT4G03500 antibody, AT4G03450 antibody, AT4G03460 antibody, AT4G03440 antibody, PIA1 antibody, AT5G15500 antibody, AT5G51160 antibody, AT5G54620 antibody, ANK6 antibody, AT1G04780 antibody, AT1G62050 antibody, Ehmt2 antibody

Background: Name/Gene ID: EHMT2

Synonyms: EHMT2, BAT8, C6orf30, Em:AF134726.3, KMT1C, G9A, GAT8, H3-K9-HMTase 3, Protein G9a, G9A histone methyltransferase, HLA-B associated transcript 8, HLA-B-associated transcript 8, Lysine N-methyltransferase 1C, NG36

Gene ID: 10919

UniProt: Q96KQ7