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Antibody
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anti-Histone H1
Histone H1 antibody
| Antigen |
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| Synonyms |
H1c, H1.2, H1f2, His1a, H1var1, MGC107646, 0610008C09Rik, LOC692942, HISTONE H1, HON4 |
| Clonality |
Monoclonal |
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Host
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Reactivity
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Application
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 Alternatives Immunocytochemistry (ICC), Western Blotting (WB), ELISA
Immunofluorescence (IF) (33), Western Blotting (WB) (30), ELISA (20), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (18), Flow Cytometry (FACS) (13), Immunohistochemistry (IHC) (10), Immunoprecipitation (IP) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Enzyme Immunoassay (EIA) (2), Immunocytochemistry (ICC) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (EM) (1)
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| Catalog no. |
ABIN211180 |
| Quantity |
200 µg (Variants) |
| Price |
700.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 10 to 15 Business days |
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Immunogen
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Cells, Nuclei from myeloid leukemia biopsy cells.
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Isotype
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IgG2a (Matching secondary antibodies)
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Description
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Gene: Histone H1
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Specificity
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Recognizes and is specific for human histone H1 at ~32-33kD. Doublet detected due to hyper-phosphorylation of histone H1 subpopulation. Cross-reacts with other histone isoforms in the following order of signal strength: H3, H2b > H4 > H2a, as determined with recombinant protein. Species cross-reactivity: bovine. Reported to stain nuclei from other animal and plant species. Predicted Reactivities: Cow (Bovine).
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Application Notes
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ICC (5 - 20 µg/ml), WB (0.5 - 2 µg/ml), ELISA
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Purification
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Protein G Column
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Buffer
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PBS, pH 7.2, 0.1% sodium azide, 40% glycerol.
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Storage
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Long term: -20°C, Short term: +4°C
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Research Area
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Chromatin and Nuclear Signaling, DNA/RNA, Transcription Factors
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Restrictions
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For Research Use only
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Alternatives for antigen "Histone H1", type "Antibodies"
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Hosts
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Rabbit (27), Mouse (18), Sheep (5), Chicken (4)
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Reactivities
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Human (40), Cow (Bovine) (22), Mouse (Murine) (18), Dog (Canine) (15), Horse (Equine) (15), Pig (Porcine) (15), Rabbit (15), Rat (Rattus) (15), Mammalian (1)
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Applications
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Immunofluorescence (IF) (33), Western Blotting (WB) (30), ELISA (20), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (18), Flow Cytometry (FACS) (13), Immunohistochemistry (IHC) (10), Immunoprecipitation (IP) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Enzyme Immunoassay (EIA) (2), Immunocytochemistry (ICC) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (EM) (1)
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Conjugates
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Alexa Flour 488 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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Acetyl-Lys25 (2), AA 100-200 (1), C-Term (1), N-Term (1), pThr17 (1)
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