MPL antibody (AA 615-628)

Catalog No. ABIN211285

Product Details anti-MPL Antibody

Target: MPL (Myeloproliferative Leukemia Virus Oncogene (MPL))

Binding Specificity: AA 615-628

Reactivity: Human, Mouse, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MPL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Specificity: Recognizes glycosylated and unglycosylated human TPO receptor/c-Mpl, Mr 70kD. Species cross-reactivity: mouse. Species sequence Homology: mouse - 100 % .

Predicted Reactivity: Percent identity by BLAST analysis: Human, Monkey, Mouse, Hamster (100%) Elephant, Rabbit (93%) Panda, Bovine, Pig (86%).

Purification: Protein A purified

Immunogen: Synthetic peptide corresponding to amino acids 615-628 of human TPO (thrombopoietin) receptor [C-ANHSYLPLSYWQQP] (KLH-conjugated). Species sequence Homology: Mouse - 100%. Percent identity by BLAST analysis: Human, Monkey, Mouse, Hamster (100%), Elephant, Rabbit (93%), Panda, Bovine, Pig (86%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IP, WB (0.25 - 1 μg/mL)

Usage: Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunohistochemistry: Stains megakaryocytes in bone marrow biopsy sections. Immunoprecipitation: 5 μg immunoprecipitates TPO receptor from 500 μg of DAMI RIPA lysate.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MPL

Target: MPL (Myeloproliferative Leukemia Virus Oncogene (MPL))

Alternative Name: CD110 / MPL (MPL Products)

Synonyms: C-MPL antibody, CD110 antibody, MPLV antibody, THCYT2 antibody, TPOR antibody, TPO-R antibody, c-mpl antibody, c-mpl-I antibody, c-mpl-II antibody, hlb219 antibody, MPL antibody, mpll antibody, tpor antibody, tpor.b antibody, MPL proto-oncogene, thrombopoietin receptor antibody, myeloproliferative leukemia virus oncogene antibody, MPL antibody, Mpl antibody, mpl antibody

Background: Name/Gene ID: MPL

Synonyms: MPL, CD110 antigen, C-MPL, CD110, MPLV, Proto-oncogene c-Mpl, TPOR, TPO-R, THCYT2, Thrombopoietin receptor

Gene ID: 4352

UniProt: P40238

Pathways: JAK-STAT Signaling