FZD10 antibody (AA 235-495)

Catalog No. ABIN2118061

Product Details anti-FZD10 Antibody

Target: FZD10 (Frizzled Family Receptor 10 (FZD10))

Binding Specificity: AA 235-495

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZD10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-WSVLCF FSSAFTVLTF LIDPSRFRYP ERPIIFLSMC YCVYSVGYII RLFAGAESIA CDRDSGQLYV IQEGLESTGC TLVFLVLYYF GMASSLWWVV LTLTWFLAAG KKWGHEAIEA NSSYFHLAAW AIPAVKTILI LVMRRVAGDE LTGVCYVGSM DVNALTGFVL VPLACYLVIG TSFILSGFVA LFHIRRVMKT GGENTDKLEK LMVRIGVFSL LYTVPATCVI ACYFYERLNM DYWKMLATQH KCKMNNQTKT PDCLM

Specificity: It has been selected for its ability to recognize FZD10 in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: FZD10 (AA 235-495)

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Comment:

Content: The quality control contains recombinant FZD10 (Trp235~Met495) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2% SDS, 200 mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details for FZD10

Target: FZD10 (Frizzled Family Receptor 10 (FZD10))

Alternative Name: Frizzled Homolog 10 (FZD10) (FZD10 Products)

Synonyms: CD350 antibody, FZ-10 antibody, Fz10 antibody, FzE7 antibody, hFz10 antibody, cFz-10 antibody, Fz-10 antibody, Xfr9 antibody, Xfz10 antibody, frizzled-10 antibody, frizzled10 antibody, fz10 antibody, fze7 antibody, hfz10 antibody, fk48e04 antibody, fz4 antibody, fzb antibody, wu:fk48e04 antibody, zg04 antibody, Xfz10A antibody, fzd10a antibody, Xfz10B antibody, Xfz9 antibody, fzd10b antibody, fzd9 antibody, frizzled class receptor 10 antibody, frizzled class receptor 10 L homeolog antibody, frizzled class receptor 10 S homeolog antibody, FZD10 antibody, fzd10 antibody, Fzd10 antibody, fzd10.L antibody, fzd10.S antibody

Pathways: WNT Signaling