Prothrombin antibody (AA 201-324)

Catalog No. ABIN5662047

Product Details anti-Prothrombin Antibody

Target: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Binding Specificity: AA 201-324

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Prothrombin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Monoclonal Antibody to Coagulation Factor II (F2)

Sequence: MGHHHHHHSG S-ANSGFLE ELRKGNLERE CVEEQCSYEE AFEALESPQD TDVFWAKYTV CDSVRKPRET FMDCLEGRCA MDLGVNYLGT VNVTHTGIQC QLWRSRYPHK PEINSTTHPG ADLKENFCRN PDSSTTGPWC YTTDPTVRRE ECSVPVCGQE GRTTVVMTPR

Specificity: The antibody is a mouse monoclonal antibody raised against F2. It has been selected for its ability to recognize F2 in immunohistochemical staining and western blotting.

Cross-Reactivity: Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Coagulation Factor II (F2) corresdonding to Ser201~Lys324 (Accession # P19221)

Clone: D4

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-5 μg/mL Immunohistochemistry: 5-30 μg/mL Immunocytochemistry: 5-30 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Prothrombin

Target: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Alternative Name: Coagulation Factor II (F2 Products)

Synonyms: PT antibody, RPRGL2 antibody, THPH1 antibody, Cf-2 antibody, Cf2 antibody, FII antibody, thrombin antibody, zgc:66319 antibody, wu:fb57c10 antibody, wu:fd42e09 antibody, wu:fd59d01 antibody, prothrombin antibody, F2 antibody, coagulation factor II, thrombin antibody, coagulation factor II antibody, coagulation factor II (thrombin) antibody, F2 antibody, f2 antibody

Background: FII, TM, PT, Thrombin, Prothrombin, Pro-Thrombin, Activation peptide fragment 1, Activation peptide fragment 2

Pathways: Complement System, Peptide Hormone Metabolism, Regulation of G-Protein Coupled Receptor Protein Signaling