Interferon gamma Protein (IFNG)

Catalog No. ABIN2136304

Product Details

Target: Interferon gamma (IFNG)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Sequence: MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC

Characteristics: Purified recombinant Mouse IFN gamma protein
Expression System: E.coli
Bioactivity: Determined by its ability to inhibit the proliferation of Mouse WEHI-279 cells. The expected ED50 is ? 0.2 ng/mL, corresponding to a specific activity of ? 5 x 10^6 units/mg.

Purity: > 98 % pure

Endotoxin Level: < 0.1 ng per μg (1 EU/μg).

Application Details

Application Notes: Each Investigator should determine their own optimal working dilution for specific applications.

Comment:

Biological activity: Determined by its ability to inhibit the proliferation of Mouse WEHI-279 cells. The expected ED50 is approx. 0.2 ng/mL, corresponding to a specific activity of approx. 5 x 10^6 units/mg.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 5-10 M Sodium Phosphate, pH 8.0 to a concentration of 1.0 mg/mL.

Buffer: Lyophilized from 10 mM Na3PO4, pH 7.0, with 20 mM NaCl.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

References

POGO, BRAWERMAN, CHARGAFF: "New ribonucleic acid species associated wihin the formation of the photosynthetic apparatus in Euglena gracilis." in: Biochemistry, Vol. 1, pp. 128-31 (PubMed).

Target Details

Target: Interferon gamma (IFNG)

Alternative Name: IFN gamma (IFNG Products)

Synonyms: IFG Protein, IFI Protein, IFN-g Protein, Ifg Protein, IFNG2 Protein, IFN-gamma Protein, IFN-G Protein, IFNG Protein, IFNgamma Protein, TCRalpha Protein, INF-G Protein, ifng Protein, interferon gamma Protein, interferon, gamma 1-2 Protein, IFNG Protein, Ifng Protein, ifng1-2 Protein

Background: IFN-gamma is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-gamma receptors are present in most immune cells, which respond to IFN-gamma signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-gamma signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulates the antigen-specific phases of the immune response. Additionally, IFN-gamma stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils.
Alternative Names: Interferon gamma protein, T cell interferon protein, IFN-g protein, type II interferon protein, IFN g protein, MAF protein, Immune Interferon protein

Molecular Weight: 15.6 kDa

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy