USP18 antibody (AA 40-49)

Catalog No. ABIN213754

Product Details anti-USP18 Antibody

Target: USP18 (Ubiquitin Specific Peptidase 18 (USP18))

Binding Specificity: AA 40-49

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP18 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Amino acids 40 to 49 of human USP18

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human USP18.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:50000), IF, IHC, IHC-P (5 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for USP18

Target: USP18 (Ubiquitin Specific Peptidase 18 (USP18))

Alternative Name: UBP43 / USP18 (USP18 Products)

Synonyms: ISG43 antibody, UBP43 antibody, bubp43 antibody, 1110058H21Rik antibody, AW047653 antibody, Ubp15 antibody, UBP antibody, USP18 antibody, USP41 antibody, ubiquitin specific peptidase 18 antibody, USP18 antibody, Usp18 antibody

Background: Name/Gene ID: USP18
Subfamily: Cysteine C19
Family: Protease

Synonyms: USP18, 43 kDa ISG15-specific protease, ISG43, HUBP43, ISG15 specific protease, Ubiquitin specific protease 18, Ubl thioesterase 18, Ubl thiolesterase 18, UBP43

Gene ID: 11274

UniProt: Q9UMW8