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EEA1 antibody (AA 307-356)

EEA1 Reactivity: Human, Dog, Rabbit, Monkey, Pig WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6736714
  • Target See all EEA1 Antibodies
    EEA1 (Early Endosome Antigen 1 (EEA1))
    Binding Specificity
    • 15
    • 6
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 307-356
    Reactivity
    • 75
    • 35
    • 28
    • 16
    • 6
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Dog, Rabbit, Monkey, Pig
    Host
    • 53
    • 17
    • 3
    • 2
    • 1
    Rabbit
    Clonality
    • 58
    • 18
    Polyclonal
    Conjugate
    • 44
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This EEA1 antibody is un-conjugated
    Application
    • 40
    • 19
    • 13
    • 13
    • 13
    • 9
    • 8
    • 7
    • 7
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human EEA1
    Predicted Reactivity
    Percent identity by BLAST analysis: Human (100%) Mouse, Guinea pig (92%) Rat, Bovine, Horse (91%) Rabbit (84%) Pig (83%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic peptide located between aa307-356 of human EEA1 (Q15075, NP_003557). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%), Galago, Mouse, Guinea pig, Platypus (92%), Rat, Bovine, Horse (91%), Elephant, Rabbit, Xenopus (84%), Bat, Pig (83%).

    Type of Immunogen: Synthetic peptide
    Isotype
    IgG
    Top Product
    Discover our top product EEA1 Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (5 μg/mL), WB (0.06 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Sterile ddH2O
    Concentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Handling Advice
    Avoid repeat freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target
    EEA1 (Early Endosome Antigen 1 (EEA1))
    Alternative Name
    EEA1 (EEA1 Products)
    Synonyms
    MST105 antibody, MSTP105 antibody, ZFYVE2 antibody, im:7151048 antibody, si:ch211-276p10.2 antibody, A430109M19Rik antibody, B230358H09Rik antibody, early endosome antigen 1 antibody, early endosome antigen 1 S homeolog antibody, EEA1 antibody, eea1 antibody, Eea1 antibody, eea1.S antibody
    Background
    Name/Gene ID: EEA1

    Synonyms: EEA1, MST105, MSTP105, ZFYVE2, Early endosome antigen 1
    Gene ID
    8411
    NCBI Accession
    NP_003557
    UniProt
    Q15075
    Pathways
    Toll-Like Receptors Cascades
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