TCF4 antibody (AA 73-122)

Catalog No. ABIN6736759

Product Details anti-TCF4 Antibody

Target: TCF4 (Transcription Factor 4 (TCF4))

Binding Specificity: AA 73-122

Reactivity: Human, Mouse, Rat, Horse, Dog, Pig, Monkey, Cow, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TCF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human TCF4

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Horse, Pig (100%) Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa73-122 of human TCF4 (P15884, NP_003190). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum (100%), Guinea pig (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from 2 % Sucrose, PBS.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TCF4

Target: TCF4 (Transcription Factor 4 (TCF4))

Alternative Name: TCF4 (TCF4 Products)

Synonyms: E2-2 antibody, ITF-2 antibody, ITF2 antibody, PTHS antibody, SEF-2 antibody, SEF2 antibody, SEF2-1 antibody, SEF2-1A antibody, SEF2-1B antibody, TCF-4 antibody, bHLHb19 antibody, 5730422P05Rik antibody, ASP-I2 antibody, E2.2 antibody, ITF-2b antibody, ME2 antibody, MITF-2A antibody, MITF-2B antibody, TFE antibody, Tcf-4 antibody, TCF7L2 antibody, XE2 antibody, Xtcf-4 antibody, Xtcf4 antibody, e2-A antibody, tcf-4 antibody, transcription factor 4 antibody, transcription factor 4 S homeolog antibody, TCF4 antibody, Tcf4 antibody, tcf4 antibody, tcf4.S antibody

Background: Name/Gene ID: TCF4
Family: Transcription factor

Synonyms: TCF4, BHLHb19, E2-2, ITF-2, ITF2, PTHS, SEF2-1B, SEF2-1, SEF2-1A, SL3-3 enhancer factor 2, SEF2, SEF-2, TCF-4, Transcription factor 4

Gene ID: 6925

NCBI Accession: NP_003190

UniProt: P15884

Pathways: WNT Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Regulation of Hormone Metabolic Process, Carbohydrate Homeostasis, Stem Cell Maintenance, Protein targeting to Nucleus