LHX1 antibody (AA 120-169)

Catalog No. ABIN6736762

Product Details anti-LHX1 Antibody

Target: LHX1 (LIM Homeobox 1 (LHX1))

Binding Specificity: AA 120-169

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Monkey, Pig, Bat, Chicken, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LHX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human LHX1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Chicken (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa120-169 of human LHX1 (P48742, NP_005559). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig, Guinea pig, Turkey, Zebra finch, Chicken (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for LHX1

Target: LHX1 (LIM Homeobox 1 (LHX1))

Alternative Name: LHX1 (LHX1 Products)

Synonyms: LIM-1 antibody, LIM1 antibody, Lim1 antibody, LHX1 antibody, lim1 antibody, wu:fe25d04 antibody, wu:fj36b06 antibody, lim-1 antibody, xlim-1 antibody, Lim-1 antibody, lmx2 antibody, LIM homeobox 1 antibody, LIM homeobox protein 1 antibody, LIM homeobox 1a antibody, LIM homeobox 1 L homeolog antibody, LHX1 antibody, Lhx1 antibody, lhx1a antibody, lhx1.L antibody

Background: Name/Gene ID: LHX1

Synonyms: LHX1, Homeobox protein Lim-1, LIM1, HLim-1, LIM homeobox 1, LIM homeobox protein 1, LIM-1, LIM/homeobox protein Lhx1

Gene ID: 3975

NCBI Accession: NP_005559

UniProt: P48742