MLF1IP antibody (pThr78)

Catalog No. ABIN214310

Product Details anti-MLF1IP Antibody

Target: MLF1IP (MLF1 Interacting Protein (MLF1IP))

Binding Specificity: pThr78

Reactivity: Human, Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MLF1IP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Brand: IHC-plus™

Specificity: Amino acids surrounding Thr78 of human MLF1IP protein.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human MLF1IP / PBIP1.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:5000 - 1:25000), IHC, IHC-P (10 μg/mL), IP, WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

Target Details for MLF1IP

Target: MLF1IP (MLF1 Interacting Protein (MLF1IP))

Alternative Name: CENPU / MLF1IP (MLF1IP Products)

Synonyms: MGC147270 antibody, CENP50 antibody, CENPU antibody, CENPU50 antibody, KLIP1 antibody, PBIP1 antibody, CENP-U antibody, CENP-50 antibody, 1700029A22Rik antibody, centromere protein U antibody, MLF1 interacting protein antibody, cenpu antibody, MLF1IP antibody, CENPU antibody, Cenpu antibody

Background: Name/Gene ID: CENPU

Synonyms: CENPU, CENP-U, CENP50, CENPU50, Centromere protein U, ICEN24, KLIP1, Polo-box-interacting protein 1, MLF1-interacting protein, CENP-50, Centromere protein of 50 kDa, MLF1 interacting protein, MLF1IP, PBIP1

Gene ID: 79682

UniProt: Q71F23