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CD4 antibody (Biotin)

CD4 Reactivity: Human FACS, IHC, IP, IF Host: Mouse Monoclonal 4AOKT4 Biotin
Catalog No. ABIN2144257
  • Target See all CD4 Antibodies
    CD4
    Reactivity
    • 355
    • 199
    • 113
    • 38
    • 24
    • 20
    • 19
    • 18
    • 15
    • 11
    • 10
    • 10
    • 8
    • 7
    • 6
    • 6
    • 6
    • 6
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 328
    • 146
    • 108
    • 4
    • 2
    • 1
    • 1
    Mouse
    Clonality
    • 445
    • 138
    • 3
    Monoclonal
    Conjugate
    • 195
    • 65
    • 54
    • 46
    • 30
    • 12
    • 11
    • 10
    • 9
    • 9
    • 8
    • 8
    • 8
    • 7
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    This CD4 antibody is conjugated to Biotin
    Application
    • 470
    • 193
    • 99
    • 93
    • 81
    • 76
    • 71
    • 42
    • 39
    • 37
    • 33
    • 24
    • 10
    • 8
    • 7
    • 7
    • 6
    • 6
    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)
    Characteristics
    CD4, also known as T4, is a 55 kD single chain transmembrane glycoprotein and belongs to immunoglobulin superfamily.
    Clone
    4AOKT4
    Isotype
    IgG2b
    Top Product
    Discover our top product CD4 Primary Antibody
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Assay Procedure
    • Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
    • Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature,
    • Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
    • Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 2 mLPBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature.
    • Repeat step 5.
    • Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
    • [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
    • [Cell fixation: 2 % formaldehyde solution]
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
  • Target
    CD4
    Alternative Name
    CD4 (CD4 Products)
    Synonyms
    cd4 antibody, L3T4 antibody, Ly-4 antibody, CD4mut antibody, W3/25 antibody, p55 antibody, CD4 molecule antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, T-cell surface glycoprotein CD4 antibody, CD4 antigen antibody, Cd4 molecule antibody, CD4 antibody, LOC100125525 antibody, cd4 antibody, Cd4 antibody
    Background
    CD4 is found on most thymocytes, a subset of T cells and at low level on monocytes/macrophages.CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction.
    Pathways
    TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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