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VCAM1 antibody (FITC)

VCAM1 Reactivity: Human FACS Host: Mouse Monoclonal 4AP8B1 FITC
Catalog No. ABIN2144710
  • Target See all VCAM1 Antibodies
    VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
    Reactivity
    • 135
    • 96
    • 55
    • 25
    • 15
    • 8
    • 8
    • 4
    • 3
    • 1
    Human
    Host
    • 96
    • 71
    • 33
    • 5
    • 1
    • 1
    Mouse
    Clonality
    • 114
    • 91
    • 1
    Monoclonal
    Conjugate
    • 94
    • 26
    • 24
    • 12
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This VCAM1 antibody is conjugated to FITC
    Application
    • 115
    • 96
    • 53
    • 42
    • 35
    • 32
    • 27
    • 26
    • 21
    • 16
    • 16
    • 13
    • 8
    • 7
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Characteristics
    CD106 is a 110 kDa single chain type I glycoprotein also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-110. It is expressed at high levels on the surface of cytokine-stimulated endothelium and at minimal levels on unstimulated endothelium.VCAM-1 serves as a ligand for the leucocyte integrins alpha 4 beta (CD49d/CD29 complex).A function of CD106 is to provide a costimulation signal in T cell activation. Reactivity with other species not tested.
    Clone
    4AP8B1
    Isotype
    IgG2a
    Top Product
    Discover our top product VCAM1 Primary Antibody
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Assay Procedure
    • Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
    • Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
    • Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
    • Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
    • [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
    • [Cell fixation: 2 % formaldehyde solution]
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
  • Target
    VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
    Alternative Name
    CD106 (VCAM1 Products)
    Synonyms
    CD106 antibody, INCAM-100 antibody, Vcam-1 antibody, VCAM1B antibody, fi76d06 antibody, wu:fi76d06 antibody, zgc:158875 antibody, VCAM1 antibody, vascular cell adhesion molecule 1 antibody, vascular cell adhesion molecule 1b antibody, VCAM1 antibody, Vcam1 antibody, vcam1b antibody, VCAM-1 antibody
    Pathways
    Carbohydrate Homeostasis
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