Collagen, Type III (COL3) antibody

Details for Product No. ABIN214580, Supplier: Log in to see
Antigen
Reactivity
Cow (Bovine), Human
161
81
64
17
9
5
3
2
1
1
1
Host
Rabbit
139
25
23
1
1
Clonality
Polyclonal
Conjugate
Un-conjugated
17
6
5
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
120
86
53
48
43
39
37
25
23
12
8
7
4
1
1
1
1
Options
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Brand IHC-plus™
Immunogen Collagen Type III from human and bovine placenta.

Type of Immunogen: Purified protein
Specificity Collagen Type III from human and bovine placenta.
Purification Immunoaffinity purified
Alternative Name Collagen III
Research Area Stem Cells, Extracellular Matrix
Application Notes Approved: ELISA (1:5000 - 1:10000), IHC-P (2.5 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.Tested in Western blot with human samples not tested with bovine.
Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer 0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA, pH 8.0, 0.01 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C
Short term: +4°C
Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Collagen, Type III (COL3) antibody (ABIN214580) Human Kidney (formalin-fixed, paraffin-embedded) stained with Collagen III antibody A...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Collagen, Type III (COL3) antibody (ABIN214580) Human Testis (formalin-fixed, paraffin-embedded) stained with Collagen III antibody A...
Product cited in: Jeong, Luo, Li, Strokes, Piao: "Characterization of G protein-coupled receptor 56 protein expression in the mouse developing neocortex." in: The Journal of comparative neurology, Vol. 520, Issue 13, pp. 2930-40, 2012 (PubMed).