CHRNB3 antibody (AA 176-225)

Catalog No. ABIN6736369

Product Details anti-CHRNB3 Antibody

Target: CHRNB3 (Cholinergic Receptor, Nicotinic, beta 3 (Neuronal) (CHRNB3))

Binding Specificity: AA 176-225

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Cow, Chicken, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRNB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human CHRNB3

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Dog, Bovine, Rabbit, Guinea pig, Chicken (100%) Horse (93%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa176-225 of human CHRNB3 (Q05901, NP_000740). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Guinea pig, Turkey, Zebra finch, Chicken (100%), Galago, Bat, Horse, Opossum (93%), Panda, Platypus (86%), Xenopus (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.03 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CHRNB3

Target: CHRNB3 (Cholinergic Receptor, Nicotinic, beta 3 (Neuronal) (CHRNB3))

Alternative Name: CHRNB3 (CHRNB3 Products)

Synonyms: 5730417K16Rik antibody, Acrb3 antibody, NACHR antibody, CHRNB3 antibody, chrnb3 antibody, cholinergic receptor nicotinic beta 3 subunit antibody, neuronal acetylcholine receptor subunit beta-3 antibody, cholinergic receptor, nicotinic, beta polypeptide 3 antibody, cholinergic receptor, nicotinic beta 3 L homeolog antibody, cholinergic receptor, nicotinic beta 3 antibody, cholinergic receptor, nicotinic, beta 3b (neuronal) antibody, cholinergic receptor, nicotinic, beta polypeptide 3a antibody, CHRNB3 antibody, CpipJ_CPIJ012638 antibody, Chrnb3 antibody, chrnb3.L antibody, chrnb3 antibody, chrnb3b antibody, chrnb3a antibody

Background: Name/Gene ID: CHRNB3
Subfamily: Nicotinic acetylcholine receptor
Family: Ion Channel

Synonyms: CHRNB3, AChR-beta3, NAChR beta 3

Gene ID: 1142

NCBI Accession: NP_000740

UniProt: Q05901