mGluR2/3 antibody (AA 871-883)

Catalog No. ABIN215162

Product Details anti-mGluR2/3 Antibody

Target: mGluR2/3 (GRIA2/3) (Glutamate Receptor, Ionotropic, AMPA 2/3 (GRIA2/3))

Binding Specificity: AA 871-883

Reactivity: Rat, Human, Mouse, Chicken, Zebrafish (Danio rerio), Horse, Monkey, Bat, Rabbit, Cow, Dog, Xenopus laevis, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This mGluR2/3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognizes both GluR2 and GluR3 which have nearly identical carboxy terminal sequences. Western blot analysis of rat brain shows two poorly separated bands which co-migrate with GluR2 and GluR3 expressed in transfected cells. GluR2 migrates slightly ahead of GluR3. No cross-reaction with GluR1 or GluR4. Species Reactivity: Human, rat, monkey, gerbil and hamster.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Opossum, Zebra finch, Chicken, Platypus, Xenopus, Pufferfish, Zebrafish (100%) Turkey, Stickleback, Trachemys (92%).

Purification: Affinity purified

Immunogen: Carboxy terminus peptide of rat GluR2 conjugated to BSA with glutaraldehyde (EGYNVYGIESVKI). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Opossum, Zebra finch, Chicken, Platypus, Xenopus, Pufferfish, Zebrafish (100%), Turkey, Stickleback, Trachemys (92%).

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-Fr, WB

Usage: Can be used for immunohistochemistry using paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Final concentrations used were 1-3 μg/mL. Western blot analysis can be done at final concentrations of 0.1-1.0 μg/mL. Labels a band at approximately 108kD. The antibody linked to immobilized Protein A immunoprecipitates detergent-solubilized receptor from brain or transfected cells.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for mGluR2/3

Target: mGluR2/3 (GRIA2/3) (Glutamate Receptor, Ionotropic, AMPA 2/3 (GRIA2/3))

Alternative Name: GLUR2 + GLUR3 (GRIA2/3 Products)

Synonyms: glutamate ionotropic receptor AMPA type subunit 2 antibody, GRIA2 antibody

Background: This antibody detects: GLUR2 + GLUR3

UniProt: P42262