Donkey anti-Goat IgG (Heavy & Light Chain) Antibody (IRDye800CW)

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Antigen
Epitope
Heavy & Light Chain
4683
2573
1882
1039
1015
825
305
273
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
1
Reactivity
Goat
2780
2760
2347
1724
1435
778
607
426
364
338
330
324
307
290
169
148
126
114
41
29
27
25
12
11
9
8
6
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
Host
Donkey
6750
4661
1833
812
684
394
88
52
51
9
8
5
5
4
2
1
1
Clonality
Polyclonal
Conjugate
IRDye800CW
2183
1862
1718
1362
765
675
521
297
295
284
274
268
217
186
178
117
102
79
69
58
54
40
35
26
23
22
19
19
19
19
19
16
15
15
15
15
15
15
14
13
12
11
11
10
10
9
9
9
9
9
9
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
In-Gel Western blotting (gelWB), Immunohistochemistry (IHC), Western Blotting (WB)
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Brand In-Cell Western™,CellTag™
Immunogen Goat IgG, whole molecule
Isotype IgG
Fragment H&L
Specificity Goat IgG
Cross-Reactivity Sheep (Ovine)
Cross-Reactivity (Details) The antibody also reacts with sheep IgG.
Characteristics IRDye 800CW Donkey anti-Goat IgG is a highly cross-adsorbed secondary antibody suitable for use in a variety of applications including Western blotting, In-Cell Western Assays, In-Gel Westerns, and many others. Based on immunoelectrophoresis, the antibody reacts with the heavy chains on goat IgG and with the light chains common to most goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with chicken, guinea pig, Syrian hamster, horse, human, mouse, rabbit, and rat serum proteins, but the antibody may cross-react with immunoglobulins from other species.
Blocking buffers and antibody diluents made with bovine serum albumin (BSA) and dry milk may contain IgG that reacts with anti-bovine IgG, anti-goat IgG, anti-horse IgG, and anti-sheep IgG antibodies. This can lead to a significant increase in background and/or reduction of secondary antibody titer for protein detection applications.
Purification immunoaffinity chromatography
Research Area Immunology, Secondary Antibodies
Application Notes Western blot: 1:5,000 - 1:25,000
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute with sterile, distilled water
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light.
Storage 4 °C
Product cited in: Li, Li, Sun, Dong, Li, Zhao, Guo, Weng, Qiu: "Poly(C)-binding protein 1, a novel N(pro)-interacting protein involved in classical swine fever virus growth." in: Journal of virology, Vol. 87, Issue 4, pp. 2072-80, 2013 (PubMed).