Donkey anti-Goat IgG (Heavy & Light Chain) Antibody (IRDye800CW)

Details for Product No. ABIN2169607, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
5027
2578
1931
1069
1007
857
330
299
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
1
Reactivity
Goat
2912
2859
2405
1752
1484
796
623
442
381
380
355
340
307
293
208
148
139
114
41
29
27
25
12
11
9
8
6
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
Host
Donkey
6893
4912
1877
895
703
401
92
54
52
21
9
8
5
5
2
1
1
Clonality
Polyclonal
Conjugate
IRDye800CW
2235
1889
1740
1374
765
679
519
300
299
288
274
268
217
188
178
124
103
79
69
60
54
46
43
40
40
36
34
32
29
29
27
27
27
27
27
27
27
27
22
22
19
19
19
19
16
15
15
15
15
15
14
12
12
11
10
10
9
9
9
9
9
9
8
8
8
8
8
7
7
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
In-Gel Western blotting (gelWB), Immunohistochemistry (IHC), Western Blotting (WB)
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Brand In-Cell Western™,CellTag™
Immunogen Goat IgG, whole molecule
Isotype IgG
Fragment H&L
Specificity Goat IgG
Cross-Reactivity Sheep (Ovine)
Cross-Reactivity (Details) The antibody also reacts with sheep IgG.
Characteristics IRDye 800CW Donkey anti-Goat IgG is a highly cross-adsorbed secondary antibody suitable for use in a variety of applications including Western blotting, In-Cell Western Assays, In-Gel Westerns, and many others. Based on immunoelectrophoresis, the antibody reacts with the heavy chains on goat IgG and with the light chains common to most goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with chicken, guinea pig, Syrian hamster, horse, human, mouse, rabbit, and rat serum proteins, but the antibody may cross-react with immunoglobulins from other species.
Blocking buffers and antibody diluents made with bovine serum albumin (BSA) and dry milk may contain IgG that reacts with anti-bovine IgG, anti-goat IgG, anti-horse IgG, and anti-sheep IgG antibodies. This can lead to a significant increase in background and/or reduction of secondary antibody titer for protein detection applications.
Purification immunoaffinity chromatography
Research Area Immunology, Secondary Antibodies
Application Notes Western blot: 1:5,000 - 1:25,000
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute with sterile, distilled water
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light.
Storage 4 °C
Product cited in: Li, Li, Sun, Dong, Li, Zhao, Guo, Weng, Qiu: "Poly(C)-binding protein 1, a novel N(pro)-interacting protein involved in classical swine fever virus growth." in: Journal of virology, Vol. 87, Issue 4, pp. 2072-80, 2013 (PubMed).