Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody (IRDye680RD)

Details for Product No. ABIN2169635, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
4683
2573
1882
1039
1015
825
305
273
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
1
Reactivity
Rabbit
2780
2760
2346
1724
1436
778
607
426
364
338
330
324
307
290
169
148
126
114
41
29
27
25
12
11
9
8
6
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
Host
Donkey
6750
4661
1833
812
684
394
88
52
51
9
8
5
5
4
2
1
1
Clonality
Polyclonal
Conjugate
IRDye680RD
2183
1862
1718
1362
765
675
521
297
295
284
274
268
217
186
178
117
102
79
69
58
54
40
35
26
23
22
19
19
19
19
19
16
15
15
15
15
15
15
14
13
12
11
11
10
10
9
9
9
9
9
9
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
In-Gel Western blotting (gelWB), Immunohistochemistry (IHC), Western Blotting (WB)
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Brand In-Cell Western™,CellTag™
Immunogen Rabbit IgG, whole molecule
Isotype IgG
Fragment Whole molecule
Specificity Rabbit IgG
Based on immunoelectrophoresis, the antibody reacts with the heavy chains on rabbit IgG and with the light chains common to most rabbit immunoglobulins.
Cross-Reactivity (Details) This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rat, and sheep serum proteins, but may cross-react with immunoglobulins from other species.
No reactivity was detected against non-immunoglobulin serum proteins.
Purification immunoaffinity chromatography
Research Area Immunology, Secondary Antibodies
Application Notes Western blot: 1:5,000 - 1:25,000
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute with sterile, distilled water
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light.
Storage 4 °C
Supplier Images
Immunoprecipitation (IP) image for Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody (IRDye680RD) (ABIN2169635) Palmitoylation of Lck kinase analyzed with a Click Chemistry IRDye® reagent. Lck was ...
Product cited in: Allan, Goggin, Caldwell: "Prenatal alcohol exposure modifies glucocorticoid receptor subcellular distribution in the medial prefrontal cortex and impairs frontal cortex-dependent learning." in: PLoS ONE, Vol. 9, Issue 4, pp. e96200, 2014 (PubMed).