Nuclear enriched lysate probed for alpha-tubulin and ELF5. The blot was probed with mouse anti-alpha-tubulin and rabbit anti-ELF5 followed by detection with IRDye® 680RD Goat anti-Mouse IgG and IRDye 800CW Goat anti-Rabbit IgG.
Dose response of EGFR phoshphorylation in A431 cells. A431 cells were treated with serial dilutions of EGF (6.3-200 ng/mL). Lysates were resolved by SDS-PAGE and transferred to Odyssey® Nitrocellulose Membrane. The blot was probed with rabbit anti-phosphoEGFR and mouse anti-ERK2 followed by detection with IRDye® 800CW Goat anti-Rabbit IgG and IRDye 680RD Goat anti-Mouse IgG.
Levels of GAD67, beta-III tubulin, and GAPDH in primary neurons. Primary neurons were genetically manipulated with a Cre-lox system to delete apoE4, and lysates were prepared. GAD67 and GAPDH were detected with mouse primary antibodies and IRDye® 800CW Goat anti-Mouse IgG (green bands. Beta-III tubulin was detected with rabbit primary and IRDye 680RD Goat anti-Rabbit IgG (red bands).