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IL-4 antibody

IL4 Reactivity: Mouse WB, ELISA, FACS, IF Host: Rat Monoclonal unconjugated
Catalog No. ABIN218112
  • Target See all IL-4 (IL4) Antibodies
    IL-4 (IL4) (Interleukin 4 (IL4))
    Reactivity
    • 135
    • 56
    • 22
    • 14
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    Mouse
    Host
    • 109
    • 59
    • 55
    • 7
    • 3
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    Rat
    Clonality
    • 125
    • 107
    • 1
    Monoclonal
    Conjugate
    • 137
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    This IL-4 antibody is un-conjugated
    Application
    • 117
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    • 15
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    Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)
    Specificity
    Recognizes mouse Interleukin-4 (IL-4).
    Purification
    Purified
    Immunogen
    E. coli-expressed recombinant mouse IL-4.
    Isotype
    IgG2b kappa
    Top Product
    Discover our top product IL4 Primary Antibody
  • Application Notes
    Approved: ELISA, Flo, IF, WB

    Usage: ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying mouse IL-4 protein levels.1-4 Purified antibody should be paired with biotinylated antibody (Cat. Immunofluorescence/. Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-4-positive cells within mixed cell populations. Recombinant mouse IL-4, unlabeled Rat IgG2b should be used as a specificity control. Western Blot: Useful for Western blot applications. For Western blotting, a concentration of 1-5 μg/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant mouse IL-4 under reducing conditions.
    Comment

    Target Species of Antibody: Mouse

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    100 mM borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added.
    Preservative
    Without preservative
    Storage
    4 °C,-20 °C
    Storage Comment
    Short term: 4°C
    Long term: Add glycerol (40-50%) -20°C.
  • Target
    IL-4 (IL4) (Interleukin 4 (IL4))
    Alternative Name
    IL4 (IL4 Products)
    Synonyms
    IL4 antibody, IL-4 antibody, BCGF-1 antibody, BCGF1 antibody, BSF-1 antibody, BSF1 antibody, Il-4 antibody, Il4e12 antibody, IL2 antibody, interleukin 4 antibody, IL4 antibody, Il4 antibody
    Background
    Name/Gene ID: IL4
    Family: Hormone

    Synonyms: IL4, B cell growth factor 1, B-cell stimulatory factor 1, B_cell stimulatory factor 1, BCGF1, BSF-1, Binetrakin, BCGF-1, Interleukin 4, Interleukin-4, Pitrakinra, BSF1, IL-4
    Gene ID
    3565
    UniProt
    P05112
    Pathways
    JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
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