Angiopoietin 1 antibody (N-Term)

Catalog No. ABIN218573

Product Details anti-Angiopoietin 1 Antibody

Target: Angiopoietin 1 (ANGPT1)

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Angiopoietin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Synthetic peptide N-Q-R-R-N-P-E-N-G-G-R-R-Y-N-R-I-Q-H-G-Q corresponding to a region (aa 21-40) near the N-terminus of mouse angiopoietin 1 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.

Purification: Sterile filtered

Immunogen: Synthetic peptide from mouse ANGPT1 / Angiopoietin-1.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:5000 - 1:25000), IHC, IHC-P (1:200), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues and mouse tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:200.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Angiopoietin 1

Target: Angiopoietin 1 (ANGPT1)

Alternative Name: ANGPT1 / ANG1 / Angiopoietin-1 (ANGPT1 Products)

Synonyms: AGP1 antibody, AGPT antibody, ANG1 antibody, 1110046O21Rik antibody, Ang-1 antibody, Ang1 antibody, Agpt antibody, Agpt1 antibody, ang1 antibody, wu:fc76g06 antibody, ANG-1 antibody, angpt1 antibody, MGC80788 antibody, angiopoietin 1 antibody, angiopoietin 1 S homeolog antibody, ANGPT1 antibody, Angpt1 antibody, angpt1 antibody, angpt1.S antibody

Background: Name/Gene ID: ANGPT1

Synonyms: ANGPT1, Angiopoietin 1, Angiopoietin-1, AGPT, AGP1, ANG-1, ANG1, KIAA0003

Gene ID: 284

UniProt: Q15389

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process