PROCR antibody

Catalog No. ABIN2192007

Product Details anti-PROCR Antibody

Target: PROCR (Protein C Receptor, Endothelial (PROCR))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This PROCR antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)

Sterility: 0.2 μm filtered

Clone: RCR-252

Application Details

Application Notes: For flow cytometry and Western blotdilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Positive RE-1 (rat 3Y1 fibroblasts stably transfected with human EPCR. control Negative N1 cells control

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

References for anti-PROCR antibody (ABIN2192007)

Bae, Yang, Rezaie: "Receptors of the protein C activation and activated protein C signaling pathways are colocalized in lipid rafts of endothelial cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 8, pp. 2867-72, (2007) (PubMed).

Finigan, Dudek, Singleton, Chiang, Jacobson, Camp, Ye, Garcia: "Activated protein C mediates novel lung endothelial barrier enhancement: role of sphingosine 1-phosphate receptor transactivation." in: The Journal of biological chemistry, Vol. 280, Issue 17, pp. 17286-93, (2005) (PubMed).

Dömötör, Benzakour, Griffin, Yule, Fukudome, Zlokovic: "Activated protein C alters cytosolic calcium flux in human brain endothelium via binding to endothelial protein C receptor and activation of protease activated receptor-1." in: Blood, Vol. 101, Issue 12, pp. 4797-801, (2003) (PubMed).

Sturn, Kaneider, Feistritzer, Djanani, Fukudome, Wiedermann: "Expression and function of the endothelial protein C receptor in human neutrophils." in: Blood, Vol. 102, Issue 4, pp. 1499-505, (2003) (PubMed).

Ye, Fukudome, Tsuneyoshi, Satoh, Tokunaga, Sugawara, Mizokami, Kimoto: "The endothelial cell protein C receptor (EPCR) functions as a primary receptor for protein C activation on endothelial cells in arteries, veins, and capillaries." in: Biochemical and biophysical research communications, Vol. 259, Issue 3, pp. 671-7, (1999) (PubMed).

Target Details for PROCR

Target: PROCR (Protein C Receptor, Endothelial (PROCR))

Alternative Name: Endothelial Protein C Receptor (PROCR Products)

Synonyms: PROCR antibody, CCCA antibody, CCD41 antibody, EPCR antibody, AI325044 antibody, Ccca antibody, Ccd41 antibody, Epcr antibody, protein C receptor antibody, protein C receptor, endothelial antibody, PROCR antibody, Procr antibody

Background: The monoclonal antibody RCR-252 recognizes human endothelial protein C receptor (EPCR), a highly glycosylated type I transmembrane protein of 221-amino-acids. These amino acids comprise an extracellular domain, a 25-aa transmembrane domain, and a short (3 aa) intracytoplasmic sequence coding for an ~46 kDa protein. Deglycosylation will reduce the protein mass to 25 kDa. EPCR is expressed strongly on the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney. EPCR is the receptor for protein C, a key player in the anticoagulation pathway. The protein C anticoagulant pathway serves as a major system for controling thrombosis, limiting inflammatory responses, and potentially decreasing endothelial cell apoptosis in response to inflammatory cytokines and ischemia. The essential components of the pathway include thrombin, thrombomodulin, the endothelial cell protein C receptor (EPCR), protein C and protein S. The pathway is initiated when thrombin binds to thrombomodulin on the surface of endothelium. EPCR augments protein C activation by binding protein C and presenting it to the thrombin-thrombomodulin activation complex. Activated protein C (aPC) retains its ability to bind EPCR, and this complex appears to be involved in some of the cellular signaling mechanisms that down-regulate inflammatory cytokine formation (TNF, IL-6). EPCR is shed from the vasculature by inflammatory mediators and thrombin. EPCR binds to activated neutrophils in a process that involves proteinase 3 and Mac-1. Furthermore, EPCR can undergo translocation from the plasma membrane to the nucleus. EPCR can be cleaved to release a soluble form (sEPCR) in the circulation. This sEPCR is detected as a single species of 43 kDa, resulting from shedding of membrane EPCR by the action of a metalloprotease, which is stimulated by thrombin and by some inflammatory mediators. Soluble EPCR binds PC and aPC with similar affinity, but its binding to aPC inhibits the anticoagulant activity of aPC by blocking its binding to phospholipids and by abrogating its ability to inactivate factor Va. sEPCR can be detected in plasma. In normal persons, sEPCR is present in levels of 83.6 +/- 17.2 ng/mL. Elevated levels of sEPCR are positively correlated to a higher risk for thrombosis. Furthermore, a haplotype (A3 allele) has been linked to elevated levels of sEPCR (264 +/-174 ng/mL). Aliases Activated protein C receptor, CD201, Endothelial cell protein C receptor Immunogen Human EPCR-positive RE-1 cells