PRTN3 antibody (FITC)

Catalog No. ABIN2192031

Product Details anti-PRTN3 Antibody (FITC)

Target: PRTN3 (Proteinase 3 (PRTN3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PRTN3 antibody is conjugated to FITC

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoassay (IA)

Sterility: 0.2 μm filtered

Clone: WGM2

Application Details

Application Notes: For flow cytometry, Western blotting and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 1 % bovine serum albumin and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References for anti-PRTN3 antibody (FITC) (ABIN2192031)

Van Der Geld, Limburg, Kallenberg: "Characterization of monoclonal antibodies to proteinase 3 (PR3) as candidate tools for epitope mapping of human anti-PR3 autoantibodies." in: Clinical and experimental immunology, Vol. 118, Issue 3, pp. 487-96, (2000) (PubMed).

Grimminger, Hattar, Papavassilis, Temmesfeld, Csernok, Gross, Seeger, Sibelius: "Neutrophil activation by anti-proteinase 3 antibodies in Wegener's granulomatosis: role of exogenous arachidonic acid and leukotriene B4 generation." in: The Journal of experimental medicine, Vol. 184, Issue 4, pp. 1567-72, (1996) (PubMed).

Braun, Csernok, Rögener-Schwarz, Ludwig, Müller-Hermelink, Gross, Feller: "Monoclonal antibody WGM1 directed against proteinase 3: an immunohistochemical marker for naphthol ASD chloroacetate." in: Hematological oncology, Vol. 14, Issue 2, pp. 83-90, (1996) (PubMed).

Braun, Csernok, Gross, Müller-Hermelink: "Proteinase 3, the target antigen of anticytoplasmic antibodies circulating in Wegener's granulomatosis. Immunolocalization in normal and pathologic tissues." in: The American journal of pathology, Vol. 139, Issue 4, pp. 831-8, (1991) (PubMed).

Csernok, Lüdemann, Gross, Bainton: "Ultrastructural localization of proteinase 3, the target antigen of anti-cytoplasmic antibodies circulating in Wegener's granulomatosis." in: The American journal of pathology, Vol. 137, Issue 5, pp. 1113-20, (1990) (PubMed).

Target Details for PRTN3

Target: PRTN3 (Proteinase 3 (PRTN3))

Alternative Name: Proteinase 3 (PRTN3 Products)

Synonyms: ela2 antibody, MGC132250 antibody, ACPA antibody, AGP7 antibody, C-ANCA antibody, CANCA antibody, MBN antibody, MBT antibody, NP-4 antibody, NP4 antibody, P29 antibody, PR-3 antibody, PR3 antibody, mPR3 antibody, proteinase 3 L homeolog antibody, proteinase 3 antibody, prtn3.L antibody, PRTN3 antibody, Prtn3 antibody

Background: Monoclonal antibody WGM2 reacts with human proteinase 3 (PR3), a 30 kDa protein. PR3 is a major antigen recognized by autoantibodies directed against cytoplasmic proteins of neutrophilic granulocytes and monocytes (so called anti-neutrophil cytoplasmic autoantibodies (ANCA)). ANCA are able to activate primed neutrophils to produce oxygen radicals and release lytic enzymes, including PR3. Proteinase 3 (PR3) was identified as the target antigen of ANCA in Wegener's granulomatosis (WG). ANCA directed against PR3 (PR3-ANCA) can interfere with the binding of PR3 to its physiological inhibitor alpha1-antitrypsin (alpha1-AT) and with the proteolytic activity of PR3. At the site of inflammation PR3 can cleave the complex between these inhibiting ANCA and PR3 itself, leaving active PR3. Autoantibodies to PR3 are potent activators of the 5-lipoxygenase pathway in primed human neutrophils. Extracellular free arachidonic acid, as present at an inflammatory focus, synergizes with such autoantibodies to evoke full-blown lipid mediator generation, granule secretion and respiratory burst. Proteinase 3 (PR3) is a neutral serine proteinase, which is localized in the azurophilic granules of neutrophils and in granules of monocytes and can be detected in the membrane of secretory vesicles. PR3 degrades a number of extracellular matrix proteins such as elastin and inactivates human C1 inhibitor. Membrane-associated PR3 is also able to activate caspase-3 without triggering apoptosis of neutrophils, which is possibly a neutrophil survival mechanism. In addition, PR3 is involved in myeloid differentiation and is, therefore, also called myeloblastin. Monoclonal antibody WGM2 blocks the PR3 activity and partially inhibits the binding of human PR3-ANCA to PR3.