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CD55 antibody

CD55 Reactivity: Rat WB, Func Host: Mouse Monoclonal RDIII-7 unconjugated
Catalog No. ABIN2192123
  • Target See all CD55 Antibodies
    CD55 (Complement Decay-Accelerating Factor (CD55))
    Reactivity
    • 110
    • 35
    • 6
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    Rat
    Host
    • 69
    • 66
    • 6
    • 6
    • 3
    • 1
    • 1
    Mouse
    Clonality
    • 81
    • 69
    • 2
    Monoclonal
    Conjugate
    • 72
    • 17
    • 16
    • 11
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD55 antibody is un-conjugated
    Application
    • 97
    • 77
    • 47
    • 39
    • 33
    • 25
    • 16
    • 15
    • 14
    • 13
    • 9
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Functional Studies (Func)
    Sterility
    0.2 μm filtered
    Clone
    RDIII-7
    Top Product
    Discover our top product CD55 Primary Antibody
  • Application Notes
    For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.
    Restrictions
    For Research Use only
  • Buffer
    PBS, containing 0.1 % bovine serum albumin.
    Storage
    4 °C
    Storage Comment
    Product should be stored at 4 °C Under recommended storage conditions, product is stable for one year.
    Expiry Date
    12 months
  • Mizuno, Harris, Morgan: "Spermatogenic cells distal to the blood-testis barrier in rats lack C3 convertase regulators and may be at risk of complement-mediated injury." in: Journal of reproductive immunology, Vol. 69, Issue 1, pp. 23-34, (2006) (PubMed).

    Mizuno, Donev, Harris, Morgan: "CD55 in rat male reproductive tissue: differential expression in testis and expression of a unique truncated isoform on spermatozoa." in: Molecular immunology, Vol. 44, Issue 7, pp. 1613-22, (2006) (PubMed).

    Harris, Williams, Linton, Morgan: "Coupling complement regulators to immunoglobulin domains generates effective anti-complement reagents with extended half-life in vivo." in: Clinical and experimental immunology, Vol. 129, Issue 2, pp. 198-207, (2002) (PubMed).

    Harris, Spiller, Morgan: "Human and rodent decay-accelerating factors (CD55) are not species restricted in their complement-inhibiting activities." in: Immunology, Vol. 100, Issue 4, pp. 462-70, (2000) (PubMed).

    Spiller, Harris, Morgan: "Efficient generation of monoclonal antibodies against surface-expressed proteins by hyperexpression in rodent cells." in: Journal of immunological methods, Vol. 224, Issue 1-2, pp. 51-60, (1999) (PubMed).

  • Target
    CD55 (Complement Decay-Accelerating Factor (CD55))
    Alternative Name
    Complement Decay Accelerating Factor (CD55 Products)
    Synonyms
    CR antibody, CROM antibody, DAF antibody, TC antibody, Daf antibody, Daf-GPI antibody, Daf1 antibody, GPI-DAF antibody, CD55 molecule (Cromer blood group) antibody, CD55 molecule, decay accelerating factor for complement antibody, CD55 antibody, Cd55 antibody
    Background
    The monoclonal antibody RDIII-7 recognizes complement decay accelerating factor (DAF), also designated as CD55. RDIII-7 recognizes the common extracellular SCR region of CD55, detecting all isoforms of the protein. Cells express on their surface several proteins which protect against complement attack, namely C receptor I (CR1), decay accelerating factor (DAF), membrane cofactor protein (MCP) and CD59. CR1, DAF and MCP regulate the activation pathways of complement by either accelerating decay of the C3 and C5 convertase (CR1, DAF), or acting as cofactors for the serine protease factor I, which cleaves and irreversibly inactivates C3b (CR1, MCP). Rat DAF (CD55) is a 60 kDa transmembrane protein that binds C3b and C4b to inhibit formation and half- life of the C3 convertases. It belongs to the receptors of complement activation (RCA) family. DAF is broadly distributed among cells in contact with plasma complment proteins, including both haematopoietic and nonhaematopoietic cells. Although DAF does not have an essential role in controlling hemolysis of erythrocytes, it has an important role in regulation of the deposition of C3 on nucleated cells. Together with other complement regulators DAF protects self cells from autologous complement- mediated injury. DAF cooperates with CD46 in circumventing autologous C3 deposition, while CD59 inhibits the pathway at the critical end-point. Aliases DAF, CD55 Immunogen NIH-3T3 cells expressing GPI-anchored rat DAF
    Pathways
    Complement System, Regulation of Leukocyte Mediated Immunity
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