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Caspase 3 antibody (Caspase 3, Apoptosis-Related Cysteine Peptidase)

Details for Product anti-CASP3 Antibody No. ABIN222869, Supplier: Log in to see
  • CC3
  • CG14902
  • Cas3
  • Casp 3
  • Casp3
  • Caspase-3
  • Decay
  • Dmel\\CG14902
  • Drosophila executioner caspase related to Apopain/Yama
  • caspase 3
  • caspase-3
  • CG7788
  • Dmel\\CG7788
  • DrICE
  • DrIce
  • Drice
  • ICE
  • crice
  • drICE
  • drIce
  • drice
  • ice
  • A830040C14Rik
  • AC-3
  • Apopain
  • CPP32
  • Lice
  • Yama
  • mldy
  • CPP32B
  • SCA-1
  • xcpp32
anti-Human Caspase 3 antibody for Western Blotting
Human, Mouse (Murine), Rat (Rattus)
Clonality (Clone)
Monoclonal ()
Western Blotting (WB)
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Immunogen Recombinant human Caspase-3
Clone C33
Isotype IgG1
Specificity The antibody recognizes both proform and the cleaved large fragment of caspase-3 in samples of human, mouse and rat origins.
Purification Affinity purified
Background The caspase family of cysteine proteases play a key role in apoptosis. Caspase-3 is the most extensively studied apoptotic protein among caspase family members. Caspase-3 is synthesized as inactive pro-enzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kD) and small (12 kD) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP and DFF).
Synonyms: CPP32 ,CASP3, apopain, procaspase3,CPP32B,SCA-1,CPP-32,Apopain,Yama
Gene ID 836
UniProt P42574
Research Area Apoptosis/Necrosis, Autophagy
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes Western blotting (0.5-4 μg/mL).
However, the optimal conditions should be determined individually.

Country of Animal Origin: USA

Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer 100 μg (200 μg/mL) in PBS containing 50 % glycerol, 0.5 mg/mL BSA and 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Caspase 3 antibody (Caspase 3, Apoptosis-Related Cysteine Peptidase) (ABIN222869) Western blot analysis of caspase-3 expression in HeLa cells treated with camptothecin...
Product cited in: Schwartz, Barker, Kaufman et al.: "Francisella tularensis inhibits the intrinsic and extrinsic pathways to delay constitutive apoptosis and prolong human neutrophil lifespan." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 7, pp. 3351-63, 2012 (PubMed).

Anand, Wilson, Hasan et al.: "Vitamin D3 enhances the apoptotic response of epithelial tumors to aminolevulinate-based photodynamic therapy." in: Cancer research, Vol. 71, Issue 18, pp. 6040-50, 2011 (PubMed).

Chiou, Hodges, Hoa: "Suppression of growth arrest and DNA damage-inducible 45alpha expression confers resistance to sulindac and indomethacin-induced gastric mucosal injury." in: The Journal of pharmacology and experimental therapeutics, Vol. 334, Issue 3, pp. 693-702, 2010 (PubMed).

El Golli Bennour, Rodriguez-Enfedaque, Bouaziz et al.: "Toxicities induced in cultured human hepatocarcinoma cells exposed to ochratoxin A: oxidative stress and apoptosis status." in: Journal of biochemical and molecular toxicology, Vol. 23, Issue 2, pp. 87-96, 2009 (PubMed).

Pietri, Caprini, Mouillet-Richard et al.: "Overstimulation of PrPC signaling pathways by prion peptide 106-126 causes oxidative injury of bioaminergic neuronal cells." in: The Journal of biological chemistry, Vol. 281, Issue 38, pp. 28470-9, 2006 (PubMed).

Atkin, Farg, Turner et al.: "Induction of the unfolded protein response in familial amyotrophic lateral sclerosis and association of protein-disulfide isomerase with superoxide dismutase 1." in: The Journal of biological chemistry, Vol. 281, Issue 40, pp. 30152-65, 2006 (PubMed).

Ahmed, Alcock, Chendil et al.: "Restoration of transforming growth factor-beta signaling enhances radiosensitivity by altering the Bcl-2/Bax ratio in the p53 mutant pancreatic cancer cell line MIA PaCa-2." in: The Journal of biological chemistry, Vol. 277, Issue 3, pp. 2234-46, 2002 (PubMed).