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Caspase 3 antibody (Caspase 3, Apoptosis-Related Cysteine Peptidase) (Cleavage Site)

Details for Product anti-CASP3 Antibody No. ABIN222873, Supplier: Log in to see
Antigen
  • CC3
  • CG14902
  • Cas3
  • Casp 3
  • Casp3
  • Caspase-3
  • DECAY
  • Decay
  • Dmel\\CG14902
  • Drosophila executioner caspase related to Apopain/Yama
  • caspase 3
  • caspase-3
  • CG7788
  • DRICE
  • Dmel\\CG7788
  • DrICE
  • DrIce
  • Drice
  • ICE
  • crice
  • drICE
  • drIce
  • drice
  • ice
  • A830040C14Rik
  • AC-3
  • Apopain
  • CPP32
  • Lice
  • Yama
  • mldy
  • CPP32B
  • SCA-1
  • xcpp32
Alternatives
anti-Human Caspase 3 antibody for Western Blotting
Epitope
Cleavage Site, N-Term, active
36
31
30
30
19
19
18
17
14
13
13
12
12
9
9
8
7
6
6
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
450
188
136
27
17
14
13
13
12
9
6
5
5
4
3
2
1
Host
Rabbit
440
66
16
3
2
Clonality
Polyclonal
Biological Activity
Active
Conjugate
This Caspase 3 antibody is un-conjugated
24
21
17
10
10
9
4
3
2
2
2
2
2
2
2
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
365
193
159
87
63
58
37
25
23
21
13
10
7
3
2
2
2
2
2
2
1
1
1
1
1
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Immunogen Synthetic peptide mapping to the N-terminus adjacent to the cleavage site of human caspase-3
Blocking Peptide Blocking peptide for this product available: ABIN412650
Isotype IgG
Specificity Preferentially recognizes active human/mouse/rat caspase-3 (p20 subunits) and does not detect the precursor form
Purification Affinity purified
Alternative Name Caspase-3 (CASP3 Antibody Abstract)
Background Caspase-3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP). The affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
Synonyms: CPP32 ,CASP3, apopain, procaspase3,CPP32B,SCA-1,CPP-32,Apopain,Yama
Gene ID 836
UniProt P42574
Research Area Apoptosis/Necrosis, Autophagy
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes Western blotting (1:50 -1:500 dilutions) and Immunohistochemistry (1:10-1:20 dilutions).
However, the optimal concentrations should be determined individually.
Comment

Country of Animal Origin: China

Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer 500 μL antigen affinity purified rabbit anti-Active Caspase-3 antibody in phosphate-buffered saline (PBS) containing 50 % glycerol, 1 % BSA, and 0.02 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Caspase 3 antibody (Caspase 3, Apoptosis-Related Cysteine Peptidase) (Cleavage Site) (ABIN222873) Western blot analysis of caspase-3 in camptothecin (2 uM) treated Jurkat cells
Product cited in: Zahavi, Ionescu, Gluska et al.: "A compartmentalized microfluidic neuromuscular co-culture system reveals spatial aspects of GDNF functions." in: Journal of cell science, Vol. 128, Issue 6, pp. 1241-52, 2015 (PubMed).

Wang, Lauer, Anand et al.: "Hyaluronan synthase 2 protects skin fibroblasts against apoptosis induced by environmental stress." in: The Journal of biological chemistry, Vol. 289, Issue 46, pp. 32253-65, 2014 (PubMed).

Anjum, Krishna, Tsutsui: "Inhibitory roles of the mammalian GnIH ortholog RFRP3 in testicular activities in adult mice." in: The Journal of endocrinology, Vol. 223, Issue 1, pp. 79-91, 2014 (PubMed).

Greenlee-Wacker, Rigby, Kobayashi et al.: "Phagocytosis of Staphylococcus aureus by human neutrophils prevents macrophage efferocytosis and induces programmed necrosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 192, Issue 10, pp. 4709-17, 2014 (PubMed).

Eslami, Sharifi, Rahimi et al.: "Protective effect of telmisartan against oxidative damage induced by high glucose in neuronal PC12 cell." in: Neuroscience letters, 2013 (PubMed).

Sun, Li, He et al.: "Neuroprotective effect of minocycline in a rat model of branch retinal vein occlusion." in: Experimental eye research, Vol. 113, pp. 105-16, 2013 (PubMed).

Finnie, Blumbergs, Manavis: "Aquaporin-4 expression after experimental contusional injury in an ovine impact-acceleration head injury model." in: Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia, Vol. 18, Issue 7, pp. 947-50, 2011 (PubMed).

Wu, Chen, Yen et al.: "Neuroprotection by the Traditional Chinese Medicine, Tao-Hong-Si-Wu-Tang, against Middle Cerebral Artery Occlusion-Induced Cerebral Ischemia in Rats." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2011, pp. 803015, 2010 (PubMed).

Krejbich-Trotot, Denizot, Hoarau et al.: "Chikungunya virus mobilizes the apoptotic machinery to invade host cell defenses." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 1, pp. 314-25, 2011 (PubMed).

Maher, Evans, Bottoms et al.: "Diminished prostaglandin E2 contributes to the apoptosis paradox in idiopathic pulmonary fibrosis." in: American journal of respiratory and critical care medicine, Vol. 182, Issue 1, pp. 73-82, 2010 (PubMed).

Ueda, Ueyama, Oka et al.: "Polaprezinc (Zinc L-carnosine) is a potent inducer of anti-oxidative stress enzyme, heme oxygenase (HO)-1 - a new mechanism of gastric mucosal protection." in: Journal of pharmacological sciences, Vol. 110, Issue 3, pp. 285-94, 2009 (PubMed).

Roy, Krishna: "Evidence of androgen-dependent sperm storage in female reproductive tract of Scotophilus heathi." in: General and comparative endocrinology, Vol. 165, Issue 1, pp. 120-6, 2009 (PubMed).

Rabin, Flitton, Demuth: "Aggregatibacter actinomycetemcomitans cytolethal distending toxin induces apoptosis in nonproliferating macrophages by a phosphatase-independent mechanism." in: Infection and immunity, Vol. 77, Issue 8, pp. 3161-9, 2009 (PubMed).

Chang, Hsieh, Peng et al.: "Neuroprotective mechanisms of puerarin in middle cerebral artery occlusion-induced brain infarction in rats." in: Journal of biomedical science, Vol. 16, pp. 9, 2009 (PubMed).

Ou, Lee, Lee et al.: "Ginkgo biloba extract attenuates oxLDL-induced oxidative functional damages in endothelial cells." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 106, Issue 5, pp. 1674-85, 2009 (PubMed).

Mendoza-Rodruiguez, Martuinez, Vargas et al.: "Mating modifies apoptosis pattern in epithelial cells of the rat uterus." in: Molecular reproduction and development, Vol. 76, Issue 6, pp. 564-72, 2009 (PubMed).

Guo, Tang, Li et al.: "[The analysis of the treatment of giant cell tumor of the pelvis and sacrum]" in: Zhonghua wai ke za zhi [Chinese journal of surgery], Vol. 46, Issue 7, pp. 501-5, 2008 (PubMed).

Frost, Zhou, Krasnesky et al.: "Initiation of oligodendrocyte progenitor cell migration by a PDGF-A activated extracellular regulated kinase (ERK) signaling pathway." in: Neurochemical research, Vol. 34, Issue 1, pp. 169-81, 2009 (PubMed).

Schmidt, Paes, De Mazière et al.: "EGFL7 regulates the collective migration of endothelial cells by restricting their spatial distribution." in: Development (Cambridge, England), Vol. 134, Issue 16, pp. 2913-23, 2007 (PubMed).

Wang, Ladrech, Pujol et al.: "Caspase inhibitors, but not c-Jun NH2-terminal kinase inhibitor treatment, prevent cisplatin-induced hearing loss." in: Cancer research, Vol. 64, Issue 24, pp. 9217-24, 2004 (PubMed).

Li, Zhang, Soledad-Conrad et al.: "Bleomycin-induced apoptosis of alveolar epithelial cells requires angiotensin synthesis de novo." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 284, Issue 3, pp. L501-7, 2003 (PubMed).