Caspase 3 antibody (Caspase 3, Apoptosis-Related Cysteine Peptidase) (Cleavage Site)

Details for Product anti-CASP3 Antibody No. ABIN222873, Supplier: Log in to see
Antigen
  • CC3
  • CG14902
  • Cas3
  • Casp 3
  • Casp3
  • Caspase-3
  • DECAY
  • Decay
  • Dmel\\CG14902
  • Drosophila executioner caspase related to Apopain/Yama
  • caspase 3
  • caspase-3
  • CG7788
  • DRICE
  • Dmel\\CG7788
  • DrICE
  • DrIce
  • Drice
  • ICE
  • crice
  • drICE
  • drIce
  • drice
  • ice
  • A830040C14Rik
  • AC-3
  • Apopain
  • CPP32
  • Lice
  • Yama
  • mldy
  • CPP32B
  • SCA-1
  • xcpp32
  • caspase 3, apoptosis-related cysteine peptidase
  • death executioner caspase related to Apopain/Yama
  • CG7788 gene product from transcript CG7788-RA
  • caspase 3
  • CASP3
  • decay
  • Ice
  • Casp3
  • casp3
Alternatives
anti-Human Caspase 3 antibody for Immunofluorescence (fixed cells)
Epitope
Cleavage Site, N-Term, active
40
32
27
24
22
21
19
19
16
15
12
12
11
10
10
9
9
7
5
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
560
305
254
48
47
38
29
28
24
20
18
18
18
12
9
8
7
4
2
2
Host
Rabbit
456
158
18
4
2
Clonality
Polyclonal
Biological Activity
Active
Conjugate
This Caspase 3 antibody is un-conjugated
27
24
19
14
9
9
9
6
4
4
4
4
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
477
237
218
142
108
78
55
50
44
29
28
13
9
9
7
5
4
3
2
2
2
2
2
2
2
1
1
1
1
Options
Supplier
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Supplier Product No.
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Available images

'Independent Validation' Badge
Antigen Caspase 3 (Cleavage Site)
Lot Number 1A273015
Method validated Immunohistochemistry
Positive Control Mouse cerebellar tissue samples at post natal day P5
Negative Control secondary antibody only
Notes The antibody works for IHC on frozen sections. Among the dilutions tested, 1:20 gave the best results. Both 1:10 and 1:50 dilutions gave a weaker signal respect to 1:20. The signal for Casp3 was detected both in cerebral cortex and cerebellum.
Secondary Antibody ABIN222873
Protocol
  • Mouse cerebellum at post natal day (P) 5 was perfusion fixed in paraformaldehyde 4% in 0.1M phosphate buffered saline (PBS) pH7.4 and post-fixed in the same fixative for 4 additional hours.
  • Following several washes in PBS, cerebellum was cryoprotected in 30% sucrose, frozen in liquid nitrogen and cut in 30μm thick sections.
  • Sections were rinsed in PBS for about 30min.
  • Sections were blocked in 0.01M PBS containing 0.1% Triton and 5% Normal Goat Serum (NGS) for 1h at RT.
  • Incubation with primary antibody ABIN222873 diluted 1:10/1:20/1:50 in the blocking solution ON at 4°C. The negative control slice was incubate with blocking solution only ON at 4°C.
  • Three 5min washed in 0.01M PBS at RT.
  • Incubation with the anti-rabbit secondary antibody (Invitrogen, AF488, Lot 1073084) diluted 1:800 in 0.1M PBS-0.1% Triton for 1h at RT.
  • Three 5min washed in 0.01M PBS at RT.
  • Specimens were mounted in Fluoroshield mounting medium (Sigma, F6182, Lot MKBX3783V).
  • Images were taken on a Leica SP5 upright confocal microscope (Magnification 40x, zoom 1.47).
Experimental Notes In order to exclude any nonspecific signal due to secondary antibody, for each experimental procedure, one control slice (negative control) was processed omitting the primary antibody: in this case the overnight incubation at 4°C degree was performed in diluent solution only.
Validation Images
Immunohistochemistry validation image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (Cleavage Site), (N-Term), (active) antibody (ABIN222873) P5 cerebellum, IHC on frozen slices 30 µm thick. Magnification 40x, zoom 1.47. A. Ant...
Immunogen Synthetic peptide mapping to the N-terminus adjacent to the cleavage site of human caspase-3
Blocking Peptide Blocking peptide for this product available: ABIN412650
Isotype IgG
Specificity Preferentially recognizes active human/mouse/rat caspase-3 (p20 subunits) and does not detect the precursor form
Purification Affinity purified
Alternative Name Caspase-3 (CASP3 Antibody Abstract)
Background Caspase-3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP). The affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
Synonyms: CPP32 ,CASP3, apopain, procaspase3,CPP32B,SCA-1,CPP-32,Apopain,Yama
Gene ID 836
UniProt P42574
Research Area Apoptosis/Necrosis, Autophagy
Pathways Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
Application Notes Western blotting (1:50 -1:500 dilutions) and Immunohistochemistry (1:10-1:20 dilutions). However, the optimal concentrations should be determined individually. The antibody preferentially recognizes the p17 fragment of the active caspase-3 in samples from human, mouse, and rat origins
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer 100 μg antigen affinity purified rabbit anti-Active Caspase-3 antibody in phosphate-buffered saline (PBS) containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Caspase 3 antibody (Caspase 3, Apoptosis-Related Cysteine Peptidase) (Cleavage Site) (ABIN222873) Western blot analysis of caspase-3 in camptothecin (2 uM) treated Jurkat cells
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