anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase)

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Antigen
Synonyms CASP9, AI115399, APAF-3, AW493809, CASP-9, Caspase-9, ICE-LAP6, Mch6, Apaf3, Casp-9-CTD, Casp9_v1, Ice-Lap6, APAF3, MCH6, PPP1R56
Alternatives
Reactivity
Cow (Bovine), Hamster, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(437), (122), (117), (15), (13), (9), (5), (4), (2), (2), (2), (1), (1), (1)
Host
Rabbit
(448), (48), (1)
Clonality
Polyclonal
Conjugate
This Caspase 9 antibody is un-conjugated
(12), (10), (7), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3)
Application
Western Blotting (WB)
(418), (187), (133), (79), (77), (62), (46), (37), (13), (12), (8), (6), (2), (2)
Pubmed 3 references available
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Quantity 100 μg
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Immunogen Synthetic peptide surrounding amino acid 290 of human caspase-9
Blocking Peptide Blocking peptide for this product available: Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) peptide (ABIN412659)
Isotype IgG
Specificity The antibody detects proform (~46 kDa), intermediate (~33-36 kDa), and the fully cleaved (~20-25 kDa) caspase-9.
Purification Affinity purified
Alternative Name Caspase-9 (CASP9 Antibody Abstract)
Background Caspase-9 is one of the most important caspases among the caspase family members. Caspase-9 is synthesized as inactive pro-enzyme that is processed in cells undergoing apoptosis. The processed caspase-9 consists of large (32-35 kD) and small (10 kD) subunits which associate to form an active enzyme. Activated caspase-9 in turn cleaves and activates caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis.
Synonyms: CASP-9 , EC 3.4.22.62 , ICE-like apoptotic protease 6 ,ICE-LAP6 ,Apoptotic protease Mch-6 ,Apoptotic protease-activating factor 3 , Apaf-3
Gene ID 842
Research Area Apoptosis/Necrosis, Cancer
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Western blotting (0.5-4 μg/mL).
However, the optimal conditions should be determined individually.
Comment

Country of Animal Origin: China

Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer 100 μg (0.2 mg/mL) affinity purified rabbit anti-caspase-9 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (ABIN222874) Western blot analysis of Caspase 9with rat kidney tissue lysate.
Product cited in: Woods, Burns, Bradford et al.: "WY-14,643 induced cell proliferation and oxidative stress in mouse liver are independent of NADPH oxidase." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 98, Issue 2, pp. 366-74, 2007 (PubMed).

Takebe, Cheng, Fandy et al.: "IMP dehydrogenase inhibitor mycophenolate mofetil induces caspase-dependent apoptosis and cell cycle inhibition in multiple myeloma cells." in: Molecular cancer therapeutics, Vol. 5, Issue 2, pp. 457-66, 2006 (PubMed).

Siu, Alway: "Mitochondria-associated apoptotic signalling in denervated rat skeletal muscle." in: The Journal of physiology, Vol. 565, Issue Pt 1, pp. 309-23, 2005 (PubMed).

Catalog No. ABIN222874
$319.00
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