Cytochrome C, Somatic (CYCS) antibody

Antigen: Cytochrome C, Somatic (CYCS)

Synonyms: cycs, CYCS, CYC, HCS, THC4, cyc, hcs, cyct, MGC75709, MGC89066, DKFZp468F1016, MGC128356, CYCSA, MGC93634, Cyt-c1, CytC-1, CytC1, Cytc, Cytc-d, DC3, bln, bs30f07.y1, cyt c-d, cyt-c-d, cyt.c, cytochrome c, ms(2)04445, pDMc02, DmelCG13263, CG13263, Cyt-c2, CytC-2, Cytc-p, DC4, DMc01, cyt c-p, cyt-c-p, l(2)SH1590, pDMc01, DmelCG17903, CG17903

Clonality: Monoclonal (7H8-2C12)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN222881

Additional Information

Alternative name: Cytochrome c

Immunogen: Pigeon cytochrome c

Format: Purified

Isotype: IgG2b

Clone: 7H8-2C12

Description: 100 ug purified mouse IgG2b (clone 7H8.2C12). Detects 12 kDa cytochrome c in denatured human/mouse/rat samples. Tested in Western blot analysis and immunoprecipitation. Synonyms: Cytochrome c Monoclonal, Anti-Cytochrome c, Cytochrome c antibody, Cytochrome c apoptosis assay kit, cytochrome c, cytochrome Synonyms: Cytochrome c Monoclonal, Anti-Cytochrome c, Cytochrome c antibody, Cytochrome c apoptosis assay kit, cytochrome c, cytochrome

Specificity: Detects 12 kDa cytochrome c in denatured human/mouse/rat samples

Synonyms: cycs, CYCS, CYC, HCS, THC4, cyc, hcs, cyct, MGC75709, MGC89066, DKFZp468F1016, MGC128356, CYCSA, MGC93634, Cyt-c1, CytC-1, CytC1, Cytc, Cytc-d, DC3, bln, bs30f07.y1, cyt c-d, cyt-c-d, cyt.c, cytochrome c, ms(2)04445, pDMc02, DmelCG13263, CG13263, Cyt-c2, CytC-2, Cytc-p, DC4, DMc01, cyt c-p, cyt-c-p, l(2)SH1590, pDMc01, DmelCG17903, CG17903

Application Details

Application Notes: The antibody can be used in Western blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody detects a 12.0 kDa protein corresponding to the apparent molecular weight of cytochrome c.

Buffer: 100 ug (0.2 mg/ml) purified IgG in PBS containing 50% glycerol, 1% BSA and 0.02% sodium azide.

Storage: Store at -20 o C. For long term storage, aliquot and refreeze at -70 o C. Avoid repeated freeze/defrost cycles Shelf Life: 12 Months

Research Area: Metabolism

Restrictions: For Research Use only

Images

Western blot anlaysis of cytochrome c with Jurkat cell lysate. Cytochrome

Publications

Saxena, Ansari, Kumar et al.: "Patulin causes DNA damage leading to cell cycle arrest and apoptosis through modulation of Bax, p(53) and p(21/WAF1) proteins in skin of mice." in: Toxicology and applied pharmacology, Vol. 234, Issue 2, pp. 192-201, 2009 (PubMed).

Zagurovskaya, Shareef, Das et al.: "EGR-1 forms a complex with YAP-1 and upregulates Bax expression in irradiated prostate carcinoma cells." in: Oncogene, Vol. 28, Issue 8, pp. 1121-31, 2009 (PubMed).

Juenemann, Weisse, Reichmann et al.: "Modulation of Mutant Huntingtin N-Terminal Cleavage and Its Effect on Aggregation and Cell Death." in: Neurotoxicity research, 2010 (PubMed).

Product: Ahmed, Alcock, Chendil et al.: "Restoration of transforming growth factor-beta signaling enhances radiosensitivity by altering the Bcl-2/Bax ratio in the p53 mutant pancreatic cancer cell line MIA PaCa-2." in: The Journal of biological chemistry, Vol. 277, Issue 3, pp. 2234-46, 2002 (PubMed).

Beaupre, Cepero, Obeng et al.: "R115777 induces Ras-independent apoptosis of myeloma cells via multiple intrinsic pathways." in: Molecular cancer therapeutics, Vol. 3, Issue 2, pp. 179-86, 2004 (PubMed).

Turpin, Lancaster, Darby et al.: "Apoptosis in skeletal muscle myotubes is induced by ceramides and is positively related to insulin resistance." in: American journal of physiology. Endocrinology and metabolism, Vol. 291, Issue 6, pp. E1341-50, 2006 (PubMed).

Machiya, Shibata, Yamauchi et al.: "Enhanced expression of MafB inhibits macrophage apoptosis induced by cigarette smoke exposure." in: American journal of respiratory cell and molecular biology, Vol. 36, Issue 4, pp. 418-26, 2007 (PubMed).

Jones, Barsby, Cohen et al.: "HIV-1 Vpr causes neuronal apoptosis and in vivo neurodegeneration." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 14, pp. 3703-11, 2007 (PubMed).

Chen, Baydoun, Xu et al.: "Lysophosphatidic acid protects mesenchymal stem cells against hypoxia and serum deprivation-induced apoptosis." in: Stem cells (Dayton, Ohio), Vol. 26, Issue 1, pp. 135-45, 2008 (PubMed).

Liang, Xu, Yin et al.: "Steroidogenic factor-1 is required for TGF-beta3-mediated 17beta-estradiol synthesis in mouse ovarian granulosa cells." in: Endocrinology, Vol. 152, Issue 8, pp. 3213-25, 2011 (PubMed).

Molouki, Yusoff: "NDV-induced apoptosis in absence of Bax; evidence of involvement of apoptotic proteins upstream of mitochondria." in: Virology journal, Vol. 9, Issue 1, pp. 179, 2012 (PubMed).