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MCL-1 antibody (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1)

Details for Product anti-MCL1 Antibody No. ABIN222889, Supplier: Log in to see
Antigen
  • AW556805
  • Mcl-1
  • BCL2L3
  • EAT
  • MCL1-ES
  • MCL1L
  • MCL1S
  • TM
  • bcl2-L-3
  • mcl1/EAT
  • mcl-1
Alternatives
anti-Human MCL-1 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Reactivity
Human, Monkey, Mouse (Murine), Rat (Rattus)
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67
9
8
4
4
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2
2
1
1
Host
Rabbit
205
76
18
2
Clonality
Polyclonal
Conjugate
This MCL-1 antibody is un-conjugated
11
9
9
6
6
5
3
2
2
2
2
2
2
2
2
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
245
118
102
58
55
31
26
22
20
8
7
6
1
Supplier
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Immunogen Synthetic peptide corresponding to residues surrounding amino acid 131 of human Mcl-1
Blocking Peptide Blocking peptide for this product available: ABIN412827
Isotype IgG
Specificity Detects 43 kDa Mcl-1
Purification Affinity purified
Alternative Name Mcl-1 (MCL1 Antibody Abstract)
Background Mcl-1 (Myeloid cell leukemia-1) is a 37 kDa Bcl-2-related protein with an apparent molecular weight of 43 kDa on SDS-PAGE. Mcl-1 was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-β1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.
Synonyms: MCL1L, mcl1/EAT, MGC1839, MGC104264, TM, MCL1S, EAT
Gene ID 4170
UniProt Q07820
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling
Application Notes Western blot analysis (0.5-4 μg/mL), Immunoprecipitation (10-20 μg/mL), and Immunohistochemistry (10-20 μg/mL).
However, the optimal conditions should be determined individually.
Comment

Country of Animal Origin: China

Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer 100 μg (0.2 mg/mL) affinity purified rabbit anti-Mcl-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-MCL-1 antibody (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1) (ABIN222889) Western blot analysis of Mcl-1 in Ramos cell lysate.
Product cited in: Xu, Wells, Padilla et al.: "A signaling pathway consisting of miR-551b, catalase and MUC1 contributes to acquired apoptosis resistance and chemoresistance." in: Carcinogenesis, Vol. 35, Issue 11, pp. 2457-66, 2014 (PubMed).

Wang, Chen, Zeng et al.: "Akt-mediated eminent expression of c-FLIP and Mcl-1 confers acquired resistance to TRAIL-induced cytotoxicity to lung cancer cells." in: Molecular cancer therapeutics, Vol. 7, Issue 5, pp. 1156-63, 2008 (PubMed).