Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) antibody

Details for Product No. ABIN222889
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Antigen
Synonyms AW556805, Mcl-1, BCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, TM, bcl2-L-3, mcl1/EAT, mcl-1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Cat (Feline)
(229), (81), (58), (17), (12), (3), (2), (2), (2), (1)
Host
Rabbit
(162), (64), (5), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(7), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB)
(192), (84), (74), (66), (53), (43), (22), (20), (19), (11), (11), (6), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN222889
297.00 $
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Immunogen Synthetic peptide corresponding to residues surrounding AA 131 of human Mcl-1
Isotype IgG
Specificity Detects 43 kDa Mcl-1
Purification Affinity purified
Alternative Name Mcl-1
Background Mcl-1 (Myeloid cell leukemia-1) is a 37 kDa Bcl-2-related protein with an apparent molecular weight of 43 kDa on SDS-PAGE. Mcl-1 was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-beta1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.
Gene ID 4170
Research Area Cancer, Apoptosis/Necrosis
Application Notes The antibody can be used for Western blot analysis (0.5-4 µg/mL).
Based on researchers feedback, it also works well in immunoprecipitation (10-20 µg/mL), and immunohistochemistry (10-20 µg/mL).
However the optimal conditions should be determined individually.
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer PBS pH 7.2, containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C/-80 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Supplier Images
anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) antibody Western blot analysis of Mcl-1 in Ramos cell lysate.
Product cited in: Wang, Chen, Zeng et al.: "Akt-mediated eminent expression of c-FLIP and Mcl-1 confers acquired resistance to TRAIL-induced cytotoxicity to lung cancer cells." in: Molecular cancer therapeutics, Vol. 7, Issue 5, pp. 1156-63, 2008 (PubMed).

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