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Heat Shock 27kDa Protein 1 (HSPB1) antibody

Details for Product No. ABIN222927
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Antigen
Synonyms
cb153, cb660, hsp1, hsp25, hsp27, id:ibd2821, sb:cb660, zgc:103437, HSPB1, cmt2f, hs.76067, hsp28, CMT2F, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27, Hs.78846, LOH11CR1K, MKBP, Hsp27, 27kDa, 2810021G ... show more
cb153, cb660, hsp1, hsp25, hsp27, id:ibd2821, sb:cb660, zgc:103437, HSPB1, cmt2f, hs.76067, hsp28, CMT2F, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27, Hs.78846, LOH11CR1K, MKBP, Hsp27, 27kDa, 2810021G24Rik show less
Reactivity
Human
(447), (105), (91), (60), (45), (24), (11), (9), (7), (6), (2), (2), (2), (1), (1), (1), (1)
Host
Rabbit
(334), (134), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(19), (19), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application
Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
(381), (169), (150), (105), (76), (65), (45), (40), (22), (22), (12), (2), (2), (2), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 µg
Options
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Catalog No. ABIN222927
324.50 $
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Immunogen Recombinant human Hsp27
Isotype IgG
Specificity Detects 27 kDa Hsp27 in human and monkey samples
Purification Affinity purified
Alternative Name Hsp27
Background Human Hsp27, mouse Hsp25 and alphabeta-crystallin are part of a diverse family of small heat shock proteins which are produced in all organisms. They function as chaperone-like proteins by binding unfolded polypeptides and preventing uncontrolled protein aggregation. Hsp27 is believed to exist mainly as oligomers of as many as 8-40 Hsp27 protein monomers in cells and data suggests that the large oligomers of Hsp27 have a chaperone-like activity by serving as a site where unfolding proteins may bind until ATP and Hsp70-dependent refolding can occur. Hsp27 is believed to protect cells by enhancing cellular glutathione levels and elevated glutathione levels have been measured in cells overexpressing Hsp27. Data from studies using wild-type Hsp27 and mutant forms in which the serine phosphorylation sites were mutated to alanines, glycines or aspartates, have shown that cellular glutathione levels depend on the oligomerization of Hsp27. Recent findings indicate that Hsp27 is also a negative regulator of cytochrome c-dependent activation of procaspase-3.
Gene ID 11036357
Research Area Cardiovascular, Contractility, Atherosclerosis, Heat Shock Proteins, Cytoskeleton, Microfilaments
Application Notes The antibody can be used in Western blotting (1-2 µg/mL), immunoprecipitation (5-10 µg/mL), immunocytochemistry (10-15 µg/mL), and ELISA (1 µg/mL).
However the optimal conditions should be determined individually.
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer PBS pH 7.2, containing 50 % glycerol, 1 % BSA, 0.02 % thimerosal
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C/-80 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Product cited in: Cheng, Pollock, Mahimkar et al.: "Matrix metalloproteinase 2 and basement membrane integrity: a unifying mechanism for progressive renal injury." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 11, pp. 1898-900, 2006 (PubMed).

Validation Images
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