Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7) (active) antibody

Details for Product No. ABIN222955
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Antigen
Synonyms xCaspase-7, caspase-7, caspase7, cmh-1, ice-lap3, mch3, CASP7, AI314680, CMH-1, ICE-IAP3, Mch3, mCASP-7, CASP-7, ICE-LAP3, LICE2, MCH3, Caspase-7, MCH3/SCA-2, SCA-2
Epitope
active
(21), (16), (10), (8), (6), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(172), (84), (63), (26), (24), (13), (12), (3), (3), (2), (1), (1), (1)
Host
Rabbit
(142), (36), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(7), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
(153), (70), (50), (44), (20), (19), (10), (9), (6), (3), (2)
Pubmed 10 references available
Quantity 100 µg
Options
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Catalog No. ABIN222955
401.50 $
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Immunogen Synthetic peptide surrounding AA 195 of human caspase-7
Isotype IgG
Specificity Preferentially recognizes the p20 subunit of active human/mouse/rat caspase-7 and does not recognize the full length caspase-7 or other caspases
Purification Affinity purified
Alternative Name Caspase-7
Background Caspase-7 has been identified as a major contributor to the execution of apoptosis. Caspase-7 is cleaved by many enzymes, including caspase-3, -6, -8, -9 and granzyme B. Active caspase-7 consists of a heterotetramer of two large and two small subunits. As an effector caspase, caspase-7 cleaves many of the same substrates as caspase-3, including poly (ADP-ribose) polymerase (PARP). The active caspase-7 antibody provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
Gene ID 840
Research Area Apoptosis/Necrosis
Application Notes The antibody can be used for Western blotting (0.5-4 µg/mL), immunoprecipitation (10-20 µg/mL), and immunohistochemistry (10-20 µg/mL).
However the optimal concentrations should be determined individually.
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer PBS containing 50 % glycerol, 1 % BSA, and 0.02 % thimerosal
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C/-80 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Supplier Images
anti-Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7) (active) antibody Western blot analysis of Jurkat cells inducedwith mM of Camptothecin for hours.
Product cited in: Scarabelli, Stephanou, Pasini et al.: "Different signaling pathways induce apoptosis in endothelial cells and cardiac myocytes during ischemia/reperfusion injury." in: Circulation research, Vol. 90, Issue 6, pp. 745-8, 2002 (PubMed).

Scarabelli, Pasini, Ferrari et al.: "Warm blood cardioplegic arrest induces mitochondrial-mediated cardiomyocyte apoptosis associated with increased urocortin expression in viable cells." in: The Journal of thoracic and cardiovascular surgery, Vol. 128, Issue 3, pp. 364-71, 2004 (PubMed).

Woods, Burns, Bradford et al.: "WY-14,643 induced cell proliferation and oxidative stress in mouse liver are independent of NADPH oxidase." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 98, Issue 2, pp. 366-74, 2007 (PubMed).

Huang, Masselli, Frisch et al.: "Blockade of tumor necrosis factor-induced Bid cleavage by caspase-resistant Rb." in: The Journal of biological chemistry, Vol. 282, Issue 40, pp. 29401-13, 2007 (PubMed).

Mahul-Mellier, Strappazzon, Petiot et al.: "Alix and ALG-2 are involved in tumor necrosis factor receptor 1-induced cell death." in: The Journal of biological chemistry, Vol. 283, Issue 50, pp. 34954-65, 2008 (PubMed).

Singh, Bond, Powell et al.: "CXCR4-gp120-IIIB interactions induce caspase-mediated apoptosis of prostate cancer cells and inhibit tumor growth." in: Molecular cancer therapeutics, Vol. 8, Issue 1, pp. 178-84, 2009 (PubMed).

Nikolaev, McLaughlin, OLeary et al.: "APP binds DR6 to trigger axon pruning and neuron death via distinct caspases." in: Nature, Vol. 457, Issue 7232, pp. 981-9, 2009 (PubMed).

Schwartz, Barker, Kaufman et al.: "Francisella tularensis inhibits the intrinsic and extrinsic pathways to delay constitutive apoptosis and prolong human neutrophil lifespan." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 7, pp. 3351-63, 2012 (PubMed).

General de Vries, Matthijsen, van Bijnen et al.: "Lysophosphatidic acid prevents renal ischemia-reperfusion injury by inhibition of apoptosis and complement activation." in: The American journal of pathology, Vol. 163, Issue 1, pp. 47-56, 2003 (PubMed).

Luyer, Derikx, Beyaert et al.: "High-fat nutrition reduces hepatic damage following exposure to bacterial DNA and hemorrhagic shock." in: Journal of hepatology, Vol. 50, Issue 2, pp. 342-50, 2009 (PubMed).

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