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Caspase 7 antibody (Caspase 7, Apoptosis-Related Cysteine Peptidase) (active)

Details for Product anti-CASP7 Antibody No. ABIN222955, Supplier: Log in to see
Antigen
  • AI314680
  • CASP-7
  • CASP7
  • Caspase-7
  • caspase-7
  • caspase7
  • cmh-1
  • CMH-1
  • ICE-IAP3
  • ice-lap3
  • ICE-LAP3
  • LICE2
  • mCASP-7
  • Mch3
  • mch3
  • MCH3
  • MCH3/SCA-2
  • SCA-2
  • xCaspase-7
Alternatives
anti-Human Caspase 7 antibody for Immunoprecipitation
Epitope
active
33
29
12
7
7
6
6
6
5
5
5
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
288
110
87
8
7
5
5
4
4
2
2
1
Host
Rabbit
203
93
4
Clonality
Polyclonal
Conjugate
This Caspase 7 antibody is un-conjugated
14
11
9
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
252
104
85
74
30
20
14
12
8
8
5
4
3
1
1
Supplier
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Immunogen Synthetic peptide surrounding amino acid 195 of human caspase-7
Isotype IgG
Specificity The antibody detects only the large subunit of cleaved caspase-7.
The antibody does not recognize full length of caspase-7 or other caspases.
Purification Affinity purified
Alternative Name Caspase-7 (CASP7 Antibody Abstract)
Background Caspase-7 has been identified as a major contributor to the execution of apoptosis. Caspase-7 is cleaved by many enzymes, including caspase-3, -6, -8, -9 and granzyme B. Active caspase-7 consists of a heterotetramer of two large and two small subunits. As an effector caspase, caspase-7 cleaves many of the same substrates as caspase-3, including poly (ADP-ribose) polymerase (PARP). The active caspase-7 antibody provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
Synonyms: CASP7,CASP-7,ICE-LAP3,CMH-1,MCH3, EC 3.4.22.60
Gene ID 840
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes Western blotting (0.5-4 μg/mL), Immunoprecipitation (10-20 μg/mL), and Immunohistochemistry (10-20 μg/mL).
However, the optimal concentrations should be determined individually.
Comment

Country of Animal Origin: China

Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer 100 μg (0.2 mg/mL) affinity purified rabbit anti-Active caspase-7 antibody in phosphate-buffered saline (PBS) containing 50 % glycerol, 1 % BSA, and 0.02 % thimerosal
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Caspase 7 antibody (Caspase 7, Apoptosis-Related Cysteine Peptidase) (active) (ABIN222955) Western blot analysis of Jurkat cells inducedwith mM of Camptothecin for hours.
Product cited in: Schwartz, Barker, Kaufman, Fayram, McCracken, Allen: "Francisella tularensis inhibits the intrinsic and extrinsic pathways to delay constitutive apoptosis and prolong human neutrophil lifespan." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 7, pp. 3351-63, 2012 (PubMed).

Luyer, Derikx, Beyaert, Hadfoune, van Kuppevelt, Dejong, Heineman, Buurman, Greve: "High-fat nutrition reduces hepatic damage following exposure to bacterial DNA and hemorrhagic shock." in: Journal of hepatology, Vol. 50, Issue 2, pp. 342-50, 2009 (PubMed).

Singh, Bond, Powell, Singh, Bumpers, Grizzle, Lillard: "CXCR4-gp120-IIIB interactions induce caspase-mediated apoptosis of prostate cancer cells and inhibit tumor growth." in: Molecular cancer therapeutics, Vol. 8, Issue 1, pp. 178-84, 2009 (PubMed).

Nikolaev, McLaughlin, OLeary, Tessier-Lavigne: "APP binds DR6 to trigger axon pruning and neuron death via distinct caspases." in: Nature, Vol. 457, Issue 7232, pp. 981-9, 2009 (PubMed).

Mahul-Mellier, Strappazzon, Petiot, Chatellard-Causse, Torch, Blot, Freeman, Kuhn, Garin, Verna, Fraboulet, Sadoul: "Alix and ALG-2 are involved in tumor necrosis factor receptor 1-induced cell death." in: The Journal of biological chemistry, Vol. 283, Issue 50, pp. 34954-65, 2008 (PubMed).

Huang, Masselli, Frisch, Hunton, Jiang, Wang: "Blockade of tumor necrosis factor-induced Bid cleavage by caspase-resistant Rb." in: The Journal of biological chemistry, Vol. 282, Issue 40, pp. 29401-13, 2007 (PubMed).

Woods, Burns, Bradford, Ross, Kosyk, Swenberg, Cunningham, Rusyn: "WY-14,643 induced cell proliferation and oxidative stress in mouse liver are independent of NADPH oxidase." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 98, Issue 2, pp. 366-74, 2007 (PubMed).

Scarabelli, Pasini, Ferrari, Ferrari, Stephanou, Lawrence, Townsend, Chen-Scarabelli, Gitti, Saravolatz, Latchman, Knight, Gardin: "Warm blood cardioplegic arrest induces mitochondrial-mediated cardiomyocyte apoptosis associated with increased urocortin expression in viable cells." in: The Journal of thoracic and cardiovascular surgery, Vol. 128, Issue 3, pp. 364-71, 2004 (PubMed).

de Vries, Matthijsen, van Bijnen, Wolfs, Buurman: "Lysophosphatidic acid prevents renal ischemia-reperfusion injury by inhibition of apoptosis and complement activation." in: The American journal of pathology, Vol. 163, Issue 1, pp. 47-56, 2003 (PubMed).

Scarabelli, Stephanou, Pasini, Comini, Raddino, Knight, Latchman: "Different signaling pathways induce apoptosis in endothelial cells and cardiac myocytes during ischemia/reperfusion injury." in: Circulation research, Vol. 90, Issue 6, pp. 745-8, 2002 (PubMed).