Perforin 1 antibody (Perforin 1 (Pore Forming Protein))

Details for Product anti-PRF1 Antibody No. ABIN222977, Supplier: Log in to see
Antigen
  • PRF1
  • LOC443187
  • perforin
  • prf1
  • FLH2
  • HPLH2
  • P1
  • PFN1
  • PFP
  • Pfn
  • Pfp
  • Prf-1
  • Cyta
  • RATCYTA
  • perforin 1 (pore forming protein)
  • perforin
  • perforin 1-like
  • PRF1
  • LOC443187
  • prf1
  • Prf1
  • LOC100342396
Alternatives
anti-Human Perforin 1 antibody for Immunohistochemistry (Frozen Sections)
Reactivity
Human
157
46
24
22
2
2
1
1
1
Host
Mouse
100
74
20
Clonality (Clone)
Monoclonal ()
Conjugate
This Perforin 1 antibody is un-conjugated
16
12
10
5
4
3
3
3
2
2
2
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC)
97
81
44
40
37
37
35
34
30
6
6
6
3
2
2
1
1
1
1
1
Options
Supplier
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Immunogen Purified granules from human YT lymphoma cells
Blocking Peptide Blocking peptide for this product available: ABIN412874
Clone DeltaG9
Isotype IgG2b
Specificity Recognizes the 70 kDa perforin in human and primate.
Purification Affinity purified
Alternative Name Perforin (PRF1 Antibody Abstract)
Background Human perforin (cytolysin) is believed to be one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis. Perforin expression is constitutive on NK cells, but increases in resting XD8+ cytotoxic cells upon activation.
Synonyms: PFP, P1 , FLH2 , HPLH2, PRF1, Cytolysin , MGC65093
Research Area Immunology, Innate Immunity, Adaptive Immunity, Inflammation
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes Immunoprecipitation (2-8 μg/mL), Flow cytometry (2-8 μg/mL), Immunohistochemistry (2-8 μg/mL for both frozen and paraffin sections), Immunocytochemistry (2-8 μg/mL),. However, the optimal conditions should be determined individually. Recognizes the 70 kDa perforin in human and primate.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer 100 μg (1 mg/mL) in PBS containing 1 mg/mL BSA and 1.5 mM thimerosal and 50 % glycerol.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Product cited in: Dulphy, Haas, Busson, Belhadj, Peffault de Latour, Robin, Carmagnat, Loiseau, Tamouza, Scieux, Rabian, Di Santo, Charron, Janin, Socié, Toubert: "An unusual CD56(bright) CD16(low) NK cell subset dominates the early posttransplant period following HLA-matched hematopoietic stem cell transplantation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 3, pp. 2227-37, 2008 (PubMed).

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