Perforin 1 (Pore Forming Protein) (PRF1) antibody

Details for Product No. ABIN222977
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Antigen
Synonyms PRF1, LOC443187, perforin, prf1, FLH2, HPLH2, P1, PFN1, PFP, Pfn, Pfp, Prf-1, Cyta, RATCYTA
Reactivity
Human
(121), (55), (31), (3), (1), (1)
Host
Mouse
(72), (70), (21)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(17), (9), (8), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
(89), (48), (38), (29), (29), (23), (21), (15), (15), (15), (10), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
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Immunogen Purified granules from human YT lymphoma cells
Clone DeltaG9
Isotype IgG2b
Purification Affinity purified
Alternative Name Perforin (PRF1 Antibody Abstract)
Background Human perforin (cytolysin) is believed to be one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis. Perforin expression is constitutive on NK cells, but increases in resting XD8+ cytotoxic cells upon activation.
Synonyms: PFP, P1 , FLH2 , HPLH2, PRF1, Cytolysin , MGC65093
Research Area Immunology, Innate Immunity, Adaptive Immunity, Inflammation
Application Notes Immunoprecipitation (2-8 µg/mL), Flow cytometry (2-8 µg/mL), Immunohistochemistry (2-8 µg/mL for both frozen and paraffin sections), Immunocytochemistry (2-8 µg/mL),.
However the optimal conditions should be determined individually.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS containing 1 mg/mL BSA and 1.5 mM thimerosal and 50 % glycerol
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C/-80 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Product cited in: Dulphy, Haas, Busson et al.: "An unusual CD56(bright) CD16(low) NK cell subset dominates the early posttransplant period following HLA-matched hematopoietic stem cell transplantation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 3, pp. 2227-37, 2008 (PubMed).

Validation Images
Catalog No. ABIN222977
352.00 $
Plus shipping costs $45.00
Quantity
Price
100 μg
352.00 $

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