BID antibody (BH3 Interacting Domain Death Agonist)

Details for Product anti-BID Antibody No. ABIN223028, Supplier: Log in to see
Antigen
  • BID
  • bid
  • si:ch211-238n5.6
  • fp497
  • xbid
  • DKFZp469E066
  • FP497
  • 2700049M22Rik
  • AI875481
  • AU022477
  • BH3 interacting domain death agonist
  • BH3 interacting domain death agonist-like
  • BH3-interacting domain death agonist-like
  • BID
  • bida
  • bid
  • Bid
  • LOC100355991
  • LOC100730546
Alternatives
anti-Human BID antibody for Immunohistochemistry (Paraffin-embedded Sections)
Reactivity
Mouse (Murine)
322
173
74
9
5
3
2
Host
Rabbit
295
96
20
9
Clonality
Polyclonal
Conjugate
This BID antibody is un-conjugated
21
19
14
9
7
7
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
348
186
127
85
54
41
33
22
13
11
11
6
3
2
1
1
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen Synthetic peptide surrounding amino acid 45 of mouse Bid
Blocking Peptide Blocking peptide for this product available: ABIN412617
Isotype IgG
Specificity The antibody detects the full length (22 kDa) mouse Bid.
Reactivity to other species has not been tested.
Purification Affinity purified
Alternative Name Bid (BID Antibody Abstract)
Background Bid, a BH3 domain-containing proapoptotic Bcl-2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In the animal model studies, Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed throμgh Fas.
Synonyms: FP497 , MGC42355, MGC15319
Gene ID 12122
UniProt P70444
Research Area Cancer, Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
Application Notes Western blot analysis (0.5-5 μg/mL) also works well in immunoprecipitation (20 μg/mL) and Immunohistochemistry (20 μg/mL). However, the optimal conditions should be determined individually. The antibody detects the full length (22 kDa) mouse Bid. Reactivity to other species has not been tested.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) affinity purified rabbit anti-Bid polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-BH3 Interacting Domain Death Agonist (BID) antibody (ABIN223028) Western blot analysis of BID expression in 3T3 cell lysate.
Product cited in: Huang, Masselli, Frisch, Hunton, Jiang, Wang: "Blockade of tumor necrosis factor-induced Bid cleavage by caspase-resistant Rb." in: The Journal of biological chemistry, Vol. 282, Issue 40, pp. 29401-13, 2007 (PubMed).

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