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Fission 1 (Mitochondrial Outer Membrane) Homolog (S. Cerevisiae) (FIS1) antibody

Details for Product No. ABIN223209, Supplier: Log in to see
Antigen
  • TTC11
  • Ttc11
  • 2010003O14Rik
Reactivity
Human, Mouse (Murine), Rat (Rattus)
54
20
20
Host
Rabbit
44
9
Clonality
Polyclonal
Conjugate
Un-conjugated
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
46
16
16
15
10
9
6
3
2
1
1
1
Supplier
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Immunogen Synthetic peptide surrounding amino acid 143 of human Fis1
Blocking Peptide Blocking peptide for this product available: ABIN412734
Isotype IgG
Specificity The antibody recognizes Fis1 of human, mouse, and rat origins.
Purification Affinity purified
Background HFis1 is a novel molecule that was discovered as part of the mammalian fission machinery. Fis 1 has been sμggested to play a role in regulation of the fission processes that might be involved in apoptotic mechanisms.
Synonyms: hFis1 , TTC11 , H_NH0132A01.6 , CGI-135
Gene ID 51024
UniProt Q9Y3D6
Application Notes Western blotting (1:100-500), Immunoprecipitation (1:50-200), Immunohistochemistry (1:100) and Immunofluorescence (1:20-100).
However, the optimal concentrations should be determined individually.
Comment

Suggested positive control: IHC: Tonsil tissue
Country of Animal Origin: China

Restrictions For Research Use only
Format Liquid
Buffer 100 μL purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 20 % glycerol, 0.2 % BSA, and 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Product cited in: Jheng, Tsai, Guo et al.: "Mitochondrial fission contributes to mitochondrial dysfunction and insulin resistance in skeletal muscle." in: Molecular and cellular biology, Vol. 32, Issue 2, pp. 309-19, 2011 (PubMed).

Thomas, McCoy, Blackinton et al.: "DJ-1 acts in parallel to the PINK1/parkin pathway to control mitochondrial function and autophagy." in: Human molecular genetics, Vol. 20, Issue 1, pp. 40-50, 2010 (PubMed).

Sandebring, Thomas, Beilina et al.: "Mitochondrial alterations in PINK1 deficient cells are influenced by calcineurin-dependent dephosphorylation of dynamin-related protein 1." in: PLoS ONE, Vol. 4, Issue 5, pp. e5701, 2009 (PubMed).

Karbowski, Neutzner, Youle: "The mitochondrial E3 ubiquitin ligase MARCH5 is required for Drp1 dependent mitochondrial division." in: The Journal of cell biology, Vol. 178, Issue 1, pp. 71-84, 2007 (PubMed).

Wasiak, Zunino, McBride: "Bax/Bak promote sumoylation of DRP1 and its stable association with mitochondria during apoptotic cell death." in: The Journal of cell biology, Vol. 177, Issue 3, pp. 439-50, 2007 (PubMed).