serpin Peptidase Inhibitor, Clade H (Heat Shock Protein 47), Member 1, (Collagen Binding Protein 1) (SERPINH1) antibody

Details for Product No. ABIN223215
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Antigen
Synonyms SERPINH1, DKFZp468B197, BERF-1, Cbp1, Cbp2, Hsp47, J6, Serpinh2, colligin, gp46, HSP47, AsTP3, CBP1, CBP2, OI10, PPROM, RA-A47, SERPINH2, astp3, cbp1, cbp2, hsp47, pig14, pprom, ra-a47, serpinh2
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(63), (32), (22), (7), (2), (2), (2), (1), (1)
Host
Rabbit
(50), (24)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Blocking Antibody (Inhibition)
(61), (28), (25), (18), (10), (8), (8), (3), (2), (2), (2), (2), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN223215
319.00 $
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Immunogen Synthetic peptide surrounding AA 407 of human HSP47
Isotype IgG
Purification Affinity purified
Alternative Name Hsp47
Background Heat shock proteins (HSPs) are ubiquitously expressed in all organisms. HSP47 is a molecule expressed constitutively in cells that synthesizes collagen. HSP47 is a procollagen-specific molecular chaperon that resides in the endoplasmic reticulum of procollagen-producing cells and is essential for secretion of procollagen from cells. Significantly, HSP47 plays a vital role in folding and assembling collagen. HSP47 is also known as colligin 1, serpinh1, collagen-binding protein 1 (CBP1) and gp46. The human HSP 47 gene encodes a 47 kDa protein.
Gene ID 12406
Research Area Signaling, Heat Shock Proteins
Application Notes The antibody can be used in Western blotting (0.5-4 µg/mL).
Based on researcher's feedback, it can also be used in immunoprecipitation (10-20 µg/mL) and immunohistochemistry (10-20 µg/mL).
However the optimal concentrations should be determined individually.
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer PBS containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C/-80 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Supplier Images
anti-serpin Peptidase Inhibitor, Clade H (Heat Shock Protein 47), Member 1, (Collagen Binding Protein 1) (SERPINH1) antibody Western blot analysis of Hsp47 expression in mouse small intestine tissue lysate.
Product cited in: Cheng, Pollock, Mahimkar et al.: "Matrix metalloproteinase 2 and basement membrane integrity: a unifying mechanism for progressive renal injury." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 11, pp. 1898-900, 2006 (PubMed).

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