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Organic Cation/carnitine Transporter4 (4-OCT) (N-Term) antibody

Details for Product No. ABIN223270, Supplier: Login to see
Antigen
Epitope
N-Term
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1
Host
Rabbit
1
Clonality
Polyclonal
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
1
1
1
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Immunogen Synthetic peptide mapping to the N-terminus of mouse Oct-4
Blocking Peptide Blocking peptide for this product available: ABIN412859
Isotype IgG
Specificity The antibody reacts with 45 kDa Oct-4 (also designated Oct-3A) and in a less extent 33 kDa Oct-3B from human, mouse, and rat samples.
Purification Affinity purified
Alternative Name 4-Oct
Background Members of Oct family of transcription factors specifically interact with Octamer motif ATGCAAAT, a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. All of the members of the Oct family contain two highly conserved domains which are separated by 14-26 variable amino acids. These include the POU homeodomain and the POU-specific domain. Both are required for DNA binding and are involved in protein-protein interactions. Evidences indicate that regulation of Oct family transcription factors occurs at the level of phosphorylation.
Synonyms: MGC22487, OCT3, OTF3, OTF4, Oct4 , POU5F1 , Oct-3
Gene ID 18999
UniProt P20263
Pathways
Application Notes Western blotting (0.5-4 μg/mL), Immunoprecipitation (20 μg/mL) and Immunohistochemistry (20 μg/mL).
Rat kidney tissue lysate can be used as a positive control.
However, the optimal conditions should be determined individually.
Comment

Country of Animal Origin: China

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) affinity purified rabbit anti-Oct-3/4 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Organic Cation/carnitine Transporter4 (4-OCT) (N-Term) antibody (ABIN223270) Western blot analysis of Oct-3/4 in rat kidney tissue lysate.
Product cited in: Josowitz, Lu, Falce et al.: "Identification and purification of human induced pluripotent stem cell-derived atrial-like cardiomyocytes based on sarcolipin expression." in: PLoS ONE, Vol. 9, Issue 7, pp. e101316, 2014 (PubMed).

Emdad, DSouza, Kothari et al.: "Efficient Differentiation of Human Embryonic and Induced Pluripotent Stem Cells into Functional Astrocytes." in: Stem cells and development, 2011 (PubMed).

Lunde, Anton, Bruusgaard et al.: "Hypoxia inducible factor 1{alpha} links fast-patterned muscle activity and fast muscle phenotype in rats." in: The Journal of physiology, 2011 (PubMed).

Carvajal-Vergara, Sevilla, DSouza et al.: "Patient-specific induced pluripotent stem-cell-derived models of LEOPARD syndrome." in: Nature, Vol. 465, Issue 7299, pp. 808-12, 2010 (PubMed).

Ho, Heydarkhan, Vernet et al.: "Stimulating Vaginal Repair in Rats Through Skeletal Muscle-Derived Stem Cells Seeded on Small Intestinal Submucosal Scaffolds." in: Obstetrics and gynecology, Vol. 114, Issue 2, Part 1, pp. 300-309, 2009 (PubMed).

Chambers, Fasano, Papapetrou et al.: "Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling." in: Nature biotechnology, Vol. 27, Issue 3, pp. 275-80, 2009 (PubMed).

Karp, Ferreira, Khademhosseini et al.: "Cultivation of human embryonic stem cells without the embryoid body step enhances osteogenesis in vitro." in: Stem cells (Dayton, Ohio), Vol. 24, Issue 4, pp. 835-43, 2006 (PubMed).