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POU Class 5 Homeobox 1 (POU5F1) (N-Term) antibody

Details for Product No. ABIN223270
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Antigen
Synonyms
POU5F1L, POU5F1, OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, NF-A3, Oct-3/4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4, gp9, gp-9, oct4, pou2, POU-2, cb197, spg/pou2, chunp6868, w ... show more
POU5F1L, POU5F1, OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, NF-A3, Oct-3/4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4, gp9, gp-9, oct4, pou2, POU-2, cb197, spg/pou2, chunp6868, wu:fd18d06 show less
Epitope
N-Term
(29), (18), (14), (10), (7), (6), (5), (5), (4), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(211), (79), (44), (29), (29), (28), (8), (7), (3), (3), (2), (2), (1)
Host
Rabbit
(153), (60), (12), (10)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
(165), (87), (46), (30), (27), (24), (21), (20), (10), (7), (4), (1)
Pubmed 7 references available
Quantity 100 µg
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Catalog No. ABIN223270
324.50 $
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Immunogen Synthetic peptide mapping to the N-terminus of mouse Oct-4
Isotype IgG
Purification Affinity purified
Alternative Name Oct-4
Background Members of Oct family of transcription factors specifically interact with Octamer motif ATGCAAAT, a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. All of the members of the Oct family contain two highly conserved domains which are separated by 14-26 variable amino acids. These include the POU homeodomain and the POU-specific domain. Both are required for DNA binding and are involved in protein-protein interactions. Evidences indicate that regulation of Oct family transcription factors occurs at the level of phosphorylation.
Gene ID 18999
UniProt Q01860
Research Area Stem Cells, Neurology
Application Notes The antibody can be used for Western blotting (0.5-4 µg/mL), immunoprecipitation (20 µg/mL) and immunohistochemistry (20 µg/mL).
However the optimal conditions should be determined individually.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C/-80 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Supplier Images
anti-POU Class 5 Homeobox 1 (POU5F1) (N-Term) antibody Western blot analysis of Oct-3/4 in rat kidney tissue lysate.
Product cited in: Karp, Ferreira, Khademhosseini et al.: "Cultivation of human embryonic stem cells without the embryoid body step enhances osteogenesis in vitro." in: Stem cells (Dayton, Ohio), Vol. 24, Issue 4, pp. 835-43, 2006 (PubMed).

Emdad, DSouza, Kothari et al.: "Efficient Differentiation of Human Embryonic and Induced Pluripotent Stem Cells into Functional Astrocytes." in: Stem cells and development, 2011 (PubMed).

Josowitz, Lu, Falce et al.: "Identification and purification of human induced pluripotent stem cell-derived atrial-like cardiomyocytes based on sarcolipin expression." in: PLoS ONE, Vol. 9, Issue 7, pp. e101316, 2014 (PubMed).

General Chambers, Fasano, Papapetrou et al.: "Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling." in: Nature biotechnology, Vol. 27, Issue 3, pp. 275-80, 2009 (PubMed).

Ho, Heydarkhan, Vernet et al.: "Stimulating Vaginal Repair in Rats Through Skeletal Muscle-Derived Stem Cells Seeded on Small Intestinal Submucosal Scaffolds." in: Obstetrics and gynecology, Vol. 114, Issue 2, Part 1, pp. 300-309, 2009 (PubMed).

Carvajal-Vergara, Sevilla, DSouza et al.: "Patient-specific induced pluripotent stem-cell-derived models of LEOPARD syndrome." in: Nature, Vol. 465, Issue 7299, pp. 808-12, 2010 (PubMed).

Lunde, Anton, Bruusgaard et al.: "Hypoxia inducible factor 1{alpha} links fast-patterned muscle activity and fast muscle phenotype in rats." in: The Journal of physiology, 2011 (PubMed).

Validation Images
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