POU Class 5 Homeobox 1 (POU5F1) antibody

Antigen: POU Class 5 Homeobox 1 (POU5F1)

Synonyms: OCT3, OCT4, OTF3, OTF4, MGC22487, POU5F1, POU5F1L

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Catalog no.: ABIN223270

Additional Information

Alternative name: Oct-4

Gene ID: 18999

UniProt: Q01860

Immunogen: Synthetic peptide mapping to the N-terminus of mouse Oct-4

Format: Purified

Description: 100 ug purified rabbit polyclonal antibody. Reacts with human/mouse/rat. Tested in Western blot/IP/IHC. Synonyms: Oct-4 Polyclonal, Anti-Oct-4 antibody, Oct-4 antibody, Anti-Oct-4, Oct-4, Oct, POU Synonyms: Oct-4 Polyclonal, Anti-Oct-4 antibody, Oct-4 antibody, Anti-Oct-4, Oct-4, Oct, POU

Application Details

Application Notes: The antibody can be used for Western blotting (0.5-4 ug/ml), immunoprecipitation (20 ug/ml) and immunohistochemistry (20 ug/ml). However, the optimal conditions should be determined individually.

Buffer: 100 ug (0.5 mg/ml) affinity purified rabbit anti-Oct-3/4 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Storage: Store at -20 o C. For long-term storage, aliquot and freeze at -70 o C. Avoid repeated freeze/defrost cycles Shelf Life: 12 Months

Research Area: Stem Cells, Neurology

Restrictions: For Research Use only

Images

Western blot analysis of Oct-3/4 in rat kidney tissue lysate.

Publications

Chambers, Fasano, Papapetrou et al.: "Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling." in: Nature biotechnology, Vol. 27, Issue 3, pp. 275-80, 2009 (PubMed).

Ho, Heydarkhan, Vernet et al.: "Stimulating Vaginal Repair in Rats Through Skeletal Muscle-Derived Stem Cells Seeded on Small Intestinal Submucosal Scaffolds." in: Obstetrics and gynecology, Vol. 114, Issue 2, Part 1, pp. 300-309, 2009 (PubMed).

Carvajal-Vergara, Sevilla, DSouza et al.: "Patient-specific induced pluripotent stem-cell-derived models of LEOPARD syndrome." in: Nature, Vol. 465, Issue 7299, pp. 808-12, 2010 (PubMed).

Lunde, Anton, Bruusgaard et al.: "Hypoxia inducible factor 1{alpha} links fast-patterned muscle activity and fast muscle phenotype in rats." in: The Journal of physiology, 2011 (PubMed).

Product: Karp, Ferreira, Khademhosseini et al.: "Cultivation of human embryonic stem cells without the embryoid body step enhances osteogenesis in vitro." in: Stem cells (Dayton, Ohio), Vol. 24, Issue 4, pp. 835-43, 2006 (PubMed).

Emdad, DSouza, Kothari et al.: "Efficient Differentiation of Human Embryonic and Induced Pluripotent Stem Cells into Functional Astrocytes." in: Stem cells and development, 2011 (PubMed).