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POU Class 5 Homeobox 1 (POU5F1) antibody
| Antigen | POU Class 5 Homeobox 1 (POU5F1) |
| Synonyms | OCT3, OCT4, OTF3, OTF4, MGC22487, POU5F1, POU5F1L |
| Clonality | Polyclonal |
| Host |
 Alternatives Rabbit |
| Reactivity |
Alternatives Human, Mouse (Murine), Rat (Rattus) |
| Application |
Alternatives Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
|
 |
6 references available |
| Catalog no. | ABIN223270 |
| Quantity | 100 ug |
| Price | 285.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 7 to 10 Business Days |
Additional Information
| Alternative name | Oct-4 |
| Gene ID | 18999 |
| Swiss-Prot | Q01860 |
| Immunogen | Synthetic peptide mapping to the N-terminus of mouse Oct-4 |
| Format | Purified |
| Description | 100 ug purified rabbit polyclonal antibody. Reacts with human/mouse/rat. Tested in Western blot/IP/IHC. Synonyms: Oct-4 Polyclonal, Anti-Oct-4 antibody, Oct-4 antibody, Anti-Oct-4, Oct-4, Oct, POU Synonyms: Oct-4 Polyclonal, Anti-Oct-4 antibody, Oct-4 antibody, Anti-Oct-4, Oct-4, Oct, POU |
Application Details
| Application Notes | The antibody can be used for Western blotting (0.5-4 ug/ml), immunoprecipitation (20 ug/ml) and immunohistochemistry (20 ug/ml). However, the optimal conditions should be determined individually. |
| Buffer | 100 ug (0.5 mg/ml) affinity purified rabbit anti-Oct-3/4 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal. |
| Storage | Store at -20 o C. For long-term storage, aliquot and freeze at -70 o C. Avoid repeated freeze/defrost cycles Shelf Life: 12 Months |
| Research Area | Stem Cells, Neurology |
| Restrictions | For Research Use only |
Publications
| Publications |
Karp, Ferreira, Khademhosseini et al.: "Cultivation of human embryonic stem cells without the embryoid body step enhances osteogenesis in vitro." in: Stem cells (Dayton, Ohio), Vol. 24, Issue 4, pp. 835-43, 2006 (PubMed).
Chambers, Fasano, Papapetrou et al.: "Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling." in: Nature biotechnology, Vol. 27, Issue 3, pp. 275-80, 2009 (PubMed). Ho, Heydarkhan, Vernet et al.: "Stimulating Vaginal Repair in Rats Through Skeletal Muscle-Derived Stem Cells Seeded on Small Intestinal Submucosal Scaffolds." in: Obstetrics and gynecology, Vol. 114, Issue 2, Part 1, pp. 300-309, 2009 (PubMed). Carvajal-Vergara, Sevilla, DSouza et al.: "Patient-specific induced pluripotent stem-cell-derived models of LEOPARD syndrome." in: Nature, Vol. 465, Issue 7299, pp. 808-12, 2010 (PubMed). Lunde, Anton, Bruusgaard et al.: "Hypoxia inducible factor 1{alpha} links fast-patterned muscle activity and fast muscle phenotype in rats." in: The Journal of physiology, 2011 (PubMed). Emdad, DSouza, Kothari et al.: "Efficient Differentiation of Human Embryonic and Induced Pluripotent Stem Cells into Functional Astrocytes." in: Stem cells and development, 2011 (PubMed). |
Alternatives
Alternatives for antigen "POU Class 5 Homeobox 1 (POU5F1)", type "Antibodies"
| Hosts | Rabbit (41), Mouse (16), Goat (7) |
| Reactivities | Human (50), Mouse (Murine) (14), Rat (Rattus) (8), Cow (Bovine) (3), Dog (Canine) (3), Chimpanzee (2), Horse (Equine) (2), Monkey (2), Pig (Porcine) (1), Primate (1) |
| Applications | Western Blotting (WB) (50), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (19), Enzyme Immunoassay (EIA) (18), ELISA (11), Immunofluorescence (IF) (5), Immunoprecipitation (IP) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Immunohistochemistry (IHC) (2), Dot Blot (Dot) (1), Immunocytochemistry (ICC) (1) |
| Epitopes | N-Term (2), AA 100-200 (1), AA 230-244 (1), E125 (1), transcript variant 1 (1) |
